How can given compounds be separated? Why was a certain method used? These are the two basic questions often asked by users of chromatography. HPLC: A Practical Guide provides the answers, by enabling the reader to grasp the concepts of the technique through simple, representative chromatograms. The book is divided into six sections, covering everything from basic concepts, instrumentation, and packing materials to the preparation, separation and technical details of the methods. Presenting the chemistry of liquid chromatography in an accessible manner, this practical guide will be useful for all those working in the field.