Fungal Protoplast A Biotechnological Tool

Contents

Part 1 Isolation of fungal protoplasts of filamentous fungi: non-engymatic procedure for the isolation of protoplasts; engymatic procedure for the isolation of protoplasts; factors affecting the release of protoplasts; monitoring fungal protoplast formation (microscopic examination); properties of the protoplasts; applications of protoplast. Part 2 Regeneration and reversion of protoplasts: regeneration and reversion; factors affecting regeneration of protoplasts; physical state of media on regeneration; other factors affecting regeneration of protoplasts; ultrastructural observations on regeneration and reversion; ultrastructure and localization of cell wall polymers during regeneration; examination of freeze-etched and negatively stained preparations of regenerating protoplasts; examination of shadowed wall preparations of regenerating protoplasts. (Part contents.)

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