Books  Animal & General Biology  Microbiology 

Microbiology Laboratory: Fundamentals and Applications

TextbookHandbook / Manual

By: George A Wistreich

688 pages, colour photos, colour illustrations

Benjamin-Cummings

Paperback | Apr 2002 | Edition: 2 | #199469 | ISBN-13: 9780130100740
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £68.99 $87/€82 approx

About this book

This comprehensive laboratory manual provides concepts, applications in microbiology, and state-of-the-art techniques. It starts with basic concepts and procedures and gradually builds to more advanced levels, strengthening students’ understanding and skills in the process.


Contents

Preface
Acknowledgments
Notes to the Student
A Short Illustrated Survey of Microbiology Laboratory Equipment
Laboratory Safety
Laboratory Safety Rules and Regulations Contract
How to Use the Laboratory Manual
Pronunciation Guide
Alphabetical Listing of Procedure Diagrams

I. AN INTRODUCTION TO MICROSCOPY AND SPECIMEN PREPARATION
Exercise 1. The Use and Care of the Microscope
Exercise 2. The Preparation of Bacterial and Oral Smears; the Use of Simple Stains
Exercise 3. Techniques for the Observation of Live Organisms: The Hanging-Drop and Temporary Wet-Mount

II. CULTIVATION TECHNIQUES
Exercise 4. Aseptic Transfer and Colony Selection Techniques
Exercise 5. Pour Plate and Streak Plate Techniques for Isolating Pure Cultures
Exercise 6. Dilution Techniques and Colony Counting
Exercise 7. A Demonstration of Bacterial Culture Characteristics
Exercise 8. Selected Techniques for the Cultivation of Anaerobes

III. A SURVEY OF THE MICROBIAL WORLD
Exercise 9. Morphological Features of Bacteria, Microscopic Measurements and Leeuwenhook's Peppercorn Water
Exercise 10. Algae and Protozoa
Exercise 11. The Fungi: Molds, Yeast, and Mushrooms
Exercise 12. The Distribution of Microorganisms in the Environment
Exercise 13. An Artificial Ecological System: The Winogradsky Column

IV. THE DIFFERENTIATION OF BACTERIAL GROUPS BY STAINING REACTIONS
Exercise 14. The Gram Stain
Exercise 15. The Acid-Fast Stain

V. BACTERIAL ANATOMY
Exercise 16. Spores
Exercise 17. Capsules
Exercise 18. Pili, Flagella, and Motility
Exercise 19. A Demonstration of Selected Bacterial Structures

VI. BIOCHEMICAL ACTIVITIES OF MICROORGANISMS
Exercise 20. A Demonstration of Selective and Differential Media
Exercise 21. Extracellular Metabolism
Exercise 22. Carbohydrate Metabolism: An Introduction to Intracellular Metabolism
Exercise 23. Nitrogen Metabolism
Exercise 24. Oxygen Utilization: Oxygen and Catalase Activities
Exercise 25. Differential Test Patterns: The IMViC Test
Exercise 26. Microbial Reactions in Multiple Test Media: Litmus Milk, Triple Sugar Iron Agar, and Sulfide Indole Motility Medium
Exercise 27. Miniaturized and Rapid Microbiological Systems
Exercise 28. An Introduction to Bacterial Identification: An Unknown Challenge

VII. THE CONTROL OF MICROORGANISMS BY CHEMICAL AND PHYSICAL FACTORS
Exercise 29. The Effectiveness of Hearing Methods on Microbial Survival
Exercise 30. The Use of Ultraviolet Radiation for Sterilization and Its Mutagenic Effects
Exercise 31. The Effects of pH on Microorganisms
Exercise 32. The Effects of Osmotic Factors on Microbial Growth
Exercise 33. The Inhibitory Action of Heavy Metals and Other Chemicals
Exercise 34. Disinfection of Selected Instruments and Equipment
Exercise 35. A Demonstration of Sterilization by Filtration and Water Pollution Detection
Exercise 36. The Bacteriostatic Activity of Dyes
Exercise 37. The Antibiogram, Antibiotic Testing, Antibiotic Resistance Detection

VIII. SELECTED ACTIVITIES OF VIRUSES
Exercise 38. Characteristics of Bacteriophages
Exercise 39. The Uses of the Embryonated Chicken Egg (Chick Embryo) and Cell Systems to Demonstrate Animal Viruses

IX. AN INTRODUCTION OF MICROBIAL GENETICS
Exercise 40. Bacterial Conjugation
Exercise 41. Rapid Bacterial Colony Transformation with Plasmid DNA
Exercise 42. Bacterial Transduction
Exercise 43. The Ames Test and the Detection of Chemical Carcinogens

X. APPLIED MICROBIOLOGY
Exercise 44. The Microbiology Examination and Content of Dairy Products and Selected Foods
Exercise 45. Cheese Making and Yogurt Production
Exercise 46. The Preparation of a Fermented Food: Sauerkraut
Exercise 47. Wine: A Product of Alcoholic Fermentation

XI. IMMUNOLOGY
Exercise 48. Microbial Agglutination Reactions
Exercise 49. Survey of Commercially Available Rapid Diagnostic Tests
Exercise 50. Agglutination: Blood Typing and Cross-Matching
Exercise 51. The Precipitin Reaction: The Ring or Interfacial, Test
Exercise 52. Gel Diffusion and Radial Immunodiffusion
Exercise 53. Enzyme Immunoassay: The Enzyme-Linked Immunosorbent Assay (ELISA)

XII. AN INTRODUCTION TO EPIDEMIOLOGY AND RELATED TOPICS
Exercise 54. Koch's Postulates in Action
Exercise 55. Universal Precautions in Action
Exercise 56. Specimen Handling and Transport
Exercise 57. Aerosolization and Its Relationship to Laboratory and Hospital Sepsis

XIII. MEDICAL MICROBIOLOGY
Exercise 58. Indigenous Flora and the Antimicrobial Activity of Body Fluids
Exercise 59. Pathogenic Microorganisms of the Skin
Exercise 60. Pathogenic Microorganisms of the Mouth, and Dental Caries
Exercise 61. Pathogens of the Respiratory Tract
Exercise 62. Pathogens of the Gastrointestinal Tract
Exercise 63. Pathogenic Microorganisms Found in Blood and Selected Microbial Pathogens of the Nervous System
Exercise 64. Pathogens of the Urogenital Tract and Sexually Transmitted Diseases (STDs)
Exercise 65. Methods for Identifying an Unknown Bacterial Specimen

XIV. AN INTRODUCTION TO PARASITOLOGY
Exercise 66. Helminthology and Selected Medically Important Arthropods

XV. EXPERIMENTAL EXERCISES
A. The Bacterial Growth Curve
B. Protein Electrophoresis
C. DNA Restriction Analysis
D. Demonstration of the Polymerase Chain Reaction (PCR)

Appendix: Answers to Critical Thinking Corners
Index


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