Functional Genomics A Practical Approach

About this book

With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialist laboratories, such as differential display and suppression subtraction hybridization. In all cases the chapters have been written by the developers of these methods or experienced users and include detailed protocols to facilitate the introduction of these methods to the readers' laboratories.

Contents

Functional Genomics: approaches and methodologies; Construction and Screening of a Subtractive cDNA Library; Differential Display Analysis of Alterations in Gene Expression; Representational Difference Analysis of cDNA; Identification of Differentially Expressed Genes by Suppression Subtractive Hybridization (SSH); Gene Expression Analysis by cDNA Microarrays; SADE: A Microassay for Serial Analysis of Gene Expression; Analysis of Gene Expression in Single Cells Using Three Prime End Amplification (TPEA) PCR; Analysis of Gene Expression by 2-D Gel Electrophoresis; Proteome Research: methods for protein characterization

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