Membrane Transport A Practical Approach

About this book

Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.

Contents

Assay of membrane transport in cells and membrane vesicles; Reconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulum; The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins; Expression of foreign transport proteins in yeast; Baculovirus-mediated overexpression of transport proteins; The amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins; Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters; Detection and analysis of glucose transporters using photolabelling techniques; Computer prediction of transporter topology and structure; Two-dimensional crystallization of membrane proteins

Customer Reviews