Enzymes in Biomass Conversion

About this book

Offering recent worldwide developments, internationally known scientists report on enzymes that are potentially important to large-scale commercial biochemical processes, including fuel and chemical feedstock production, pulp and paper processing, waste degradation and processing, and food processing. Chapters cover basic knowledge of what enzymes are available, what their properties are, and how best to use them. Describes specific enzyme applications, methods of enzyme production, and characteristics of individual enzymes. Among the methods for producing and recovering enzymes described are enzyme cloning, liquid fermentation, enzyme immobilization, and enzyme cross-linking. Also presents alternate viewpoints on the question of lignin degradation and other controversial subjects.

Contents

Enzymes for Fuels and Chemical Feedstocks; Enzymes in Pulp and Paper Processing; Enzymes for Anaerobic Municipal Solid Waste Disposal; Thermostable Saccharidases: New Sources, Uses, and Biodesigns; Alkali-Enzymes Produced by an Alkaliphilic Bacillus Strain: Mannan-Degrading Enzymes Produced by Bacillus sp. AM-001; Proteinases and Their Inhibitors in Biotechnology; Subtilisin: Commercially Relevant Model for Large-Scale Enzyme Production; Enzymes from Solid Substrates: Recovering Extracellular Degradative Enzymes from Lenticula edodes Cultures Grown on Commercial Wood Medium; Production of Trichoderma reesei Cellulase System with High Hydrolytic Potential by Solid-State Fermentation; Role of Statistically Designed Experiments in the Development of Efficient Downstream Processes; Enhanced Utility of Polysaccharidases through Chemical Cross-linking and Immobilization Applications; Bioprocessing Aids in the Recovery of Proteins from Biomass; Chromatography in Enzyme Isolation and Production; Lignin Peroxidase: Catalysis, Oxycomplex, and Heme-Linked Ionization; Structure and Regulation of Manganese Peroxidase Gene from Phanerochaete chrysosporium; Regulation of Ligninase Production in White-Rot Fungi; Laccases of the Ligninolytic Fungus; Pilot-Scale Production and Properties of Lignin Peroxidases; Chemistry of Lignin Degradation: An Extracurricular View; Lignin-Carbohydrate Complexes from Polar Wood: Isolation and Enzymatic Degradation; Cellulase: Insights through Recombinant DNA Approaches; Structure of Cellulolytic Enzymes; Thermal Unfolding of Trichoderma reesei CBH I; Bacterial Cellulases; Regulation of Synthesis; Cellulomonas fimi *b-1,4-Glucanases; Comparison of Amylopullulanase to *a-Amylase and Pullulanase; Cyclodextrin Glucanotransferases: Technology and Biocatalysis Design; Starch Liquefacation with a Highly Thermostable Cyclodextrin Glycosyl Transferase from Thermoanaerobacter sp; Reactions of Glucansucrases in the Biomass Conversion of Sucrose; Biotechnological Potential and Production of Xylanolytic Systems Free of Cellulases; Catalytic Properties and Partial Amino Acid Sequence of an Actinomycete Endo-(1RT4)-*b-D-Xylanase from Chainia sp; Accessory Enzymes Involved in the Hydrolysis of Xylans; Comparison of Endolytic Hydrolases That Depolymerize 1,4-*b-D-Mannan, 1,5-*a-L-Arabinan, and 1,4-*b-D-Galactan; Microbial Strategies for the Depolymerization of Plant and Algal Polyuronates; Synergism between 1,3-*b-Glucanases in Yeast Cell Wall Zymolysis; Chitinases

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