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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Enzymes of Molecular Biology

Handbook / Manual
Series: Methods in Molecular Biology Volume: 16
Edited By: M Burrell
384 pages, Illus
Publisher: Humana Press
Enzymes of Molecular Biology
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  • Enzymes of Molecular Biology ISBN: 9780896032347 Spiralbound May 1993 Not in stock: Usually dispatched within 1-2 weeks
    £89.99
    #33181
Price: £89.99
About this book Contents Customer reviews Related titles

About this book

Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols.

Contents

Nucleases: An Overview. Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1). DNA Polymerases (EC 2.7.7.7). Taq Polymerases (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Protocols. Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6). Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase to Synthesize DNA from RNA. Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31). Restriction Enzymes. DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73). DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3). The BAL 31 Nucleases (EC 3.1.11). Mung-Bean Nuclease 1 (EC 3.1.30.1). RNase A (EC 3.1.27.5). Pronase (EC 3.4.24.4). Proteolytic Enzymes for Peptide Production. Proteinase K (EC 3.4.21.14). Carboxypeptidase Y (EC 3.4.16.1). Aminopeptidases: Aminopeptidase M (EC 3.4.11.2), Pyroglutamate Aminopeptidase (EC 3.4.19.3), and Prolidase (EC 3.4.13.9). Alkaline Phosphatase (EC 3.1.3.1). Polynucleotide Kinase (EC 2.7.1.78). Index.

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 16
Edited By: M Burrell
384 pages, Illus
Publisher: Humana Press
Media reviews
An essential resource for every biochemist and scientist in molecular biology. - Unlisted Drugs
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