Microarray technology provides a highly sensitive and precise technique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be rapidly analyzed. The protocols collected in this important volume provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological sample for analysis. Opening with an overview from Professor Southern on the history of DNA array technology, the reader is provided with an all-encompassing manual of the latest state-of-the-art techniques including descriptions of unique ways to synthesize arrays using the photolithographic approach, the synthesis and use of modified monomers, genotyping using DNA Mass Array methodology, as well as information on antisense reagents, a future drug market that will be used to study the effects of these molecules by using array hybridization. Also covered are the business aspects of biochip technology as well as the ethical issues related to genetic analysis. Comprehensive and state-of-the-art, this book will be useful to all levels of scientists from novices to those intimately familiar with array technology.
DNA Arrays - Methods and Protocols presents microarray technology as an extremely useful tool to better understand modern molecular biology, particularly DNA sequencing, gene expression, genetic mutation and polymorphism. The main focus is on a very detailed presentation of the available methods in microarray technology. Experiments are explained carefully and systematic attention is paid to possible variations and particular difficulties that may arise. Useful tips are offered: as to how to perform direct comparisons of gene-expression patterns between two tissues or two different experimental condition this book will surely be viewed as one that represented a stepping stone into development of the technique. - Department of Chemistry, University of Toronto DNA Arrays: Methods and Protocols is both a useful introduction to DNA microarray technology as well as a handy source of protocols for specific applications of the technique. The book covers a broad range of topics related to DNA microarrays beginning of DNA arrays to the analysis of the data that is produced. This book offers many practical insights into DNA microarray analysis that should prove to be useful to scientists interested in implementing this technique in their research. the reader should be able to easily follow the progression of the material through the different stages one would need to perform to design a microarray experiment. This book will be of interest for those readers seeking practical information about DNA array methodologies. - Biotech Software & Internet Report of interest to postgraduate students and postdoctoral researchers who have begun work on DNA arrays and need a broader view of the field, together with useful tips for solving practical problems. - Angewente Chemie International
DNA Microarrays: History and Overview, Edwin M. Southern. Gel-Immobilized Microarrays of Nucleic Acids and Proteins: Production and Application for Macromolecular Research, Jordanka Zlatanova and Andrei Mirzabekov. Sequencing by Hybridization Arrays, Radoje Drmanac and Snezana Drmanac. Ethical Ramifications of Genetic Analysis Using DNA Arrays, Wayne W. Grody. Photolithographic Synthesis of High-Density Oligonucleotide Arrays, Glenn H. McGall and Jacqueline A. Fidanza. Automated Genotyping Using the DNA MassArrayTM Technology, Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Koster. Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules, Patrick Cooley, Debra Hinson, Hans-Jochen Trost, Bogdan Antohe, and David Wallace. Printing DNA Microarrays Using the Biomek(R) 2000 Laboratory Automation Workstation, David W. Galbraith, Jiri Macas, Elizabeth A. Pierson, Wenying Xu, and Marcela Nouzova. Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays, Ulrich Certa, Antoine de Saizieu, and Jan Mous. Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays, Rogelio Maldonado-Rodriguez and Kenneth L. Beattie. DNA Sequencing by Hybridization with Arrays of Samples or Probes, Radoje Drmanac, Snezana Drmanac, Joerg Baier, Gloria Chui, Dan Coleman, Robert Diaz, Darryl Gietzen, Aaron Hou, Hui Jin, Tatjana Ukrainczyk, and Chongjun Xu. Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents, Muhammad Sohail and Edwin M. Southern. Low-Resolution Typing of HLA-DQA1 Using DNA Microarray, Sarah H. Haddock, Christine Quartararo, Patrick Cooley, and Dat D. Dao. Gene Expression Analysis on Medium-Density Oligonucleotide Arrays, Ralph Sinibaldi, Catherine O'Connell, Chris Seidel, and Henry Rodriguez. Use of Bioinformatics in Arrays, Peter Kalocsai and Soheil Shams. Confocal Scanning of Genetic Microarrays, Arthur E. Dixon and Savvas Damaskinos. Business Aspects of Biochip Technologies, Kenneth E. Rubenstein. Index.
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