Tissue Culture Techniques An Introduction

Contents

Biology of the living cell - cell organelles and reactions, the cell cycle; sterility - aseptic technique, sterilization methods, quality control of sterilization, exercises; cell culture - feeding schedules and media components, subcultivation, cell enumeration and cell viability, putting routine methods to work, detecting and disposing of contamination, troubleshooting, safety, problem sets, exercises; culture manipulations - alterations of the media, substrata, altering the environment, problem set, exercises; primary cell culture - isolation, characterization; cell preservation and cloning - cell banking, cell cloning, problem set, exercises; cell transformation and transfection - cell transformation and immortality, transfection, exercises; finding information for new cell work - data bases; advertising. Appendices: basic laboratory equipment; solutions; vendors; problem set answers.

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