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About this book
Contents
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About this book
Contains 10 chapters that cover the key kinetic methods currently applied to measure the functions and dynamics of proteins and nucleic acids.
Contents
1. Introduction to kinetic analysis of enzyme systems; 2. Detection and characterisation of enzyme intermediates; 3. Kinetic analysis of DNA-Protein Interactions by time-resolved synchrotron X-ray footprinting; 4. Kinetic analysis of ribozyme cleavage; 5. Transient-state kinetics and computational analysis of transcription initiation; 6. A fluorescent sensor to assay inorganic phosphate; 7. What to do if there is no signal; 8. The biotin regulatory system-kinetic, themodynamic and structural interrogation of a biological switch; 9. Time resolved spectral analysis; 10. Kinetic analysis of enzyme mutants for identification of catalytic residues
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