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This lab manual is designed to give students experience with a wide variety of model systems currently in use by developmental biologists. Experiments range from classic slide or whole animal observations to more modern immunohistochemistry and manipulation of gene expression.
TABLE OF CONTENTS; THE EXPERIMENTS; Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems; Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls; Plant Cell Totipotency: Growing a Carrot from Adult Cells; Fertilization: Sea Urchin Syngamy & Development; Early Plant Development: Pollen Tube Formation; Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye; Cell Adhesion: Cell-Cell Interactions in Sponges & Amphibians; Embryogenesis: Chick & Amphibian Development (Slide-based); Neurulation: Labelling Chick Notochord using Immunocytochemistry; Gametogenesis: Comparison of Plants and Animals; Regeneration: Morphallaxis & Epimorphosis; Metamorphosis: Drosphila Imaginal Discs; External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish; INFORMATION FOR THE INSTRUCTOR; Animal Care & Maintenance; Animal & Plant Sources; Chemical Sources; Slides Needed / Sources; Solutions & Other Materials; GLOSSARY; LITERATURE CITED
All in all a clearly written laboratory aid that should prove highly useful to those involved in developmental biology. Biologist (2004) 51 (3) Am I recommending it to collegues? Yes, because adpating the practicals described here is a good way to bring unfamiliar organisms into the classroom and can be used to let students explore points made in our own, different, courses. If you want to read about simple, tested developmental protocols for sponges, planaria, sea urchins, killfish, amphibians, chicks, beans and pondweed, this is a good place to start. BioEssays 26: 1142
