704 pages, 427 illus
In the decade following the publication of the human genome sequence, we have witnessed a revolution in recombinant DNA technology and its applications. The use of gene manipulation techniques on an ever-increasing scale has underpinned numerous advances in biotechnology and medicine, and has vastly increased our understanding of the world around us.
Now in its eighth edition, "Principles of Gene Manipulation and Genomics" embraces this revolution, providing integrated coverage of the techniques used for gene manipulation, genomics, and its related disciplines. As in previous editions, the text covers the basic gene manipulation techniques and then describes recent developments in recombinant DNA technology, genome analysis, functional genomics, and proteomics, bringing these themes together in a final section that discusses how the techniques are applied in the real world.The eighth edition includes a number of new features such as full-color illustrations throughout, color-coded text boxes in each chapter offering insights into the history, methodology, ethical concerns, and applications of genomic technologies, chapter summaries and thought-provoking end-of-chapter questions.
The book is accompanied by a dedicated website providing further instruction and resources for both the student and instructor as well as regular updates on important topics. All art from the book is available to instructors for download from the website. "Principles of Gene Manipulation and Genomics" is an accessible and up-to-date guide to this exciting field, and is the essential reference for upper-level undergraduate and graduate students of genetics, genomics, molecular biology, and recombinant DNA technology.
As with previous texts written by Primrose, the subjects are discussed in enough detail to stimulate first to final-year biology and genetics undergraduates and will also help to remind research students about some of the basics they have forgotten. - Times Higher Education Supplement
List of plates.Preface.Abbreviations.1 Gene manipulation in the post-genomics era.Part I Fundamental Techniques of Gene Manipulation.2 Basic techniques.3 Cutting and joining DNA molecules.4 Basic biology of plasmid and phage vectors.5 Cosmids, phasmids, and other advanced vectors.6 Gene-cloning strategies.7 Sequencing genes and short stretched of DNA.8 Changing genes: site-directed mutagenesis and protein engineering.9 Bioinformatics.Part II Manipulating DNA in Microbes, Animals, and Plants.10 Cloning in bacteria other than Escherichia coli.11 Cloning in Saccharomyces cerevisiae and other fungi.12 Gene transfer to animal cells.13 Genetic manipulation of animals.14 Gene transfer to plants.15 Advanced transgenic technology.Part III Genome Analysis, Genomics, and Beyond.16 The organization and structure of genomes.17 Mapping and sequencing genomes.18 Comparative genomics.19 Large-scale mutagenesis and interference.20 Analysis of the transcriptome.21 Proteomics I - Expression analysis and characterization of proteins.22 Proteomics II - Analysis of protein structures.23 Proteomics III - Protein interactions.24 Metabolomics and global biochemical networks.Part IV Applications of Gene Manipulations and Genomics.25 Applications of genomics: understanding the basis of polygenic disorders and identifying quantitative trait loci.26 Applications of recombinant DNA technology.Glossary.References.Index
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Sandy Primrose was an academic for 10 years and a senior manager in the biotechnology industry for 20 years. He now manages a consultancy practice focused on technology management. Richard Twyman was an academic for 9 years and now manages a company that provides expert scientific writing and project management services. He is also a Visiting Fellow at the Department of Biology, University of York.