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Germ Cell Protocols: Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning

Handbook / Manual

Series: Methods in Molecular Biology Volume: 254

Edited By: Heidi Schatten

Humana Press

Hardback | Dec 2004 | #147837 | ISBN: 1588292576
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £119.50 $152/€143 approx

About this book

For volume 2 alone: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems. For both volumes: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.


Contents

Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos Michele Boiani, James Kehler, and Hans R. Scholer Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans Monika A. Jedrusik and Ekkehard Schulze cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development Myriam Hemberger PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells: Cloning Uniquely Expressed RNAs from Rare Cells Thiagarajan Venkataraman, Elio Dancausse, and Mary Lou King Differentiating Embryonic Stem Cells into Embryoid Bodies Gisele Hopfl, Max Gassmann, and Isabelle Desbaillets The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation Edward J. Gallagher and Jim McWhir Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos Jayne M. Squirrell and John G. White Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes Stephen A. Stricker A Method for Producing Cloned Pigs by Using Somatic Cells as Donors Liangxue Lai and Randall S. Prather Pronuclear Microinjection of Mouse Zygotes Thomas Rulicke Parthenogenesis and Nuclear Transfer in Rabbit Oocytes Yukio Tsunoda and Yoko Kato Nuclear Transfer in Cattle Gottfried Brem and Manzoor A. Nowshari In Vitro Fertilization and Embryo Transfer in Felids Charles Earle Pope Production of Chimeric Chickens Mitsuru Naito and Takashi Kuwana Production of Haploid and Diploid Androgenetic Zebrafish Bruce P. Brandhorst and Graham E. Corley-Smith Construction and Detection of Fluorescent, Germline Transgenic Zebrafish Elwood Linney and Ava J. Udvadia Production of Zebrafish Germline Chimeras from Cultured Cells Lianchun Fan, Annette Alestrom, Peter Alestrom, and Paul Collodi Studying Nuclear Reprogramming with Cell Hybrids Steve Pells and Jim McWhir Cytoplasmic Transfer in Mammalian Eggs and Embryos Henry E. Malter Conventional Technologies for Cryopreservation of Human Oocytes and Embryos Michael J. Tucker, Sharon Shipley, and Juergen Liebermann Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts: Vitrification Procedures as an Alternative to Conventional Cryopreservation Juergen Liebermann, and Michael J. Tucker Microfluidic Removal of Cumulus Cells from Mammalian Zygotes Henry C. Zeringue and David J. Beebe Mammalian Embryo Culture in a Microfluidic Device Eric M. Walters, Sherrie G. Clark, David J. Beebe, and Matthew B. Wheeler Application of Alginate Gels to the Study of Mammalian Development Richard L. Gardner Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos Steven F. Mullen and John K. Critser Index

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