Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
Technical Considerations in Quantitating Gene Expression. Gene Expression Quantitation Technology Summary. Standardized RT-PCR and the Standardized Expression Measurement Center. GeneCalling: Transcript Profiling Coupled to a Gene Database Query. Invader Assay for RNA Quantitation. Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays. Amplified Differential Gene Expression Microarray. Suppression Subtractive Hybridization. Small Amplified RNA-SAGE. Gene Expression Informatics. Index.
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