Recombinant Gene Expression Reviews and Protocols

About this book

In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Contents

Table of Contents; Recombinant Gene Expression: Reviews and Protocols; Second Edition; Edited by Paulina Balbas and Argelia Lorence; Part1. General Issues About Recombinant Gene Expression; 1 Host Cell Compatibility in Protein Expression - James J. Greene; 2 Production of Recombinant Proteins: Challenges and Solutions - Laura A. Palomares, Sandino Estrada Moncada, and Octavio T. Ramirez; 3 Folding Promoting Agents in Recombinant Protein Production: A Review Beatrix Fahnert; Part2. Prokaryotes; 4 Back to Basics: pBR322 and Protein Expression Systems in E. coli. - Paulina Balbas and Francisco Bolivar; 5 Alpha-Complementation Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments - Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson; 6 Expression of Recombinant Alkaline Phosphatase Conjugates in E. coli - Jean-Claude Boulain and Frederic Ducancel; 7 Overexpression of chromosomal genes in E. coli - Fernando Valle and Noemi Flores; 8 Chromosomal Expression of Foreign and Native Genes from Regulatable Promoters in E. coli - Lu Zhou, Ke Zhang and Barry L. Wanner; 9 Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in coli - Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset; 10 Copy-Control pBAC/oriV Vectors For Genomic Cloning - Jadwiga Wild and Waclaw Szybalski; 11 Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV - Jadwiga Wild and Waclaw Szybalski; 12 Cell-Free Protein Synthesis with Coupled Transcription and Translation - James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford; 13 Genetic Tools For The Manipulation of Moderately Halophilic Bacteria of The Family Halomonadaceae - Carmen Vargas and Joaquin J. Nieto; 14 Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria - Amalia S. Afendra, Carmen Vargas, Joaquin J. Nieto, and Constantin Drainas; 15 Recombinant Protein Production in Antarctic Gram-Negative Bacteria - Angela Duilio, Maria Luisa Tutino, and Gennaro Marino; Part3. Fungi; 16 Recombinant Protein Production in Yeasts - Danilo Porro and Diethard Mattanovich; 17 Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae - Kevin J. Vestrepen and Johan M. Thevelein; 18 High Throughput Expression in Microplate Formate in Saccharomyces cerevisiae - Caterina Holz and Christine Lang; 19 High Throughput Expression in Microplate Formate in Pichia pastoris - Mewes Botner and Christine Lang; 20 Multiple Gene Expression by Chromosomal Integration and CRE-loxP Mediated Marker Recycling in Saccharomyces cerevisiae - Bjorn Johansson and Barbel Hahn-Hagerdal; 21 Fungal Transformation: Key Concepts and Applications - Vianey Olmedo-Monfil, Carlos Cortes-Penagos, and Alfredo Herrera-Estrella; 22 Three Decades of Fungal Transformation: The Technologies - Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella; Part4: Plants And Plant

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