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Genetic recombination is any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. This book presents leading peer-reviewed protocols to carry out recombinant investigations in the lab. Part I provides approaches and model systems for studying several aspects of recombination in a variety of eukaryotic organisms and in mammalian parasites. Part II describes approaches for using recombination as a reporter of genomic instability in lower and higher eukaryotes. Part III discusses various methods and approaches for targeted genomic manipulation in higher and lower eukaryotes, while the final part presents biochemical analyses useful for furthering the understanding of recombination mechanisms, thus making this an invaluable book for both the novice and the established researcher in the field of recombination.
Part I. Studying Recombination Events in Eukaryotes Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae Rachelle Miller Spell and Sue Jinks-Robertson Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells Jason A. Smith and Alan S. Waldman Intrachromosomal Homologous Recombination in Arabidopsis thaliana Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease Jac A. Nickoloff and Mark A. Brenneman Transformation of Monomorphic and Pleomorphic Trypanosoma brucei Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima Part II. Recombination as a Reporter of Genomic Instability Detecting Carcinogens With the Yeast DEL Assay Richard J. Brennan and Robert H. Schiestl In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl Part III. Recombination as a Tool for Producing Targeted Genetic Modification Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms Mark D. Baker DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis Targeted Gene Modification Using Triplex-Forming Oligonucleotides Jean Y. Kuan and Peter M. Glazer Using Nucleases to Stimulate Homologous Recombination Dana Carroll Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA Katie K. Maguire and Eric B. Kmiec Part IV. Biochemistry of Recombination Chromatin Immunoprecipitation to Investigate Protein DNA Interactions During Genetic Recombination Tamara Goldfarb and Eric Alani Holliday Junction Branch Migration and Resolution Assays Angelos Constantinou and Stephen C. West Index
