This is a diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods for studying B-lymphocyte development and function in higher animals. These readily reproducible protocols follow the successful "Methods in Molecular Biology" series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen Richard R. Hardy and Susan A. Shinton Characterization of B-Cell Maturation in the Peripheral Immune System Rita Carsetti Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, and Leonore A. Herzenberg Analysis of B-Cell Life-Span and Homeostasis Irmgard Forster Differentiation of B Lymphocytes From Hematopoietic Stem Cells Paulo Vieira and Ana Cumano Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System Faith M. Young, Carl A. Pinkert, and Andrea Bottaro Conditional Gene Mutagenesis in B-Lineage Cells Stefano Casola Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze T-Cell-Dependent Immune Responses, Germinal Center Development, and the Anaysis of V-Gene Sequences Claudia Berek Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors Kuo-I Lin and Kathryn Calame Immunoglobulin Class Switching: In Vitro Induction and Analysis Sven Kracker and Andreas Radbruch In Vitro and In Vivo Assays of B-Lymphocyte Migration Chantal Moratz and John H. Kehrl Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization Tilman Brummer, Winfried Elis, Michael Reth, and Michael Huber Isolation of Lipid Rafts From B Lymphocytes Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder, and Susan K. Pierce Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells Ralf Kuppers Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice Helen Ferry and Richard J. Cornall Analysis of B-Cell Signaling Using DT40 B-Cell Line Tomoharu Yasuda and Tadashi Yamamoto B-Cell Development and Pre-B-1 Cell Plasticity In Vitro Antonius G. Rolink Index
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