Books  Animal & General Biology  Biochemistry & Molecular Biology 

Principles and Reactions of Protein Extraction, Purification, and Characterization

By: H Ahmed

416 pages, 160 b/w illus

CRC Press

Spiralbound | Dec 2004 | #151820 | ISBN: 0849320348
Availability: Usually dispatched within 6 days Details
NHBS Price: £105.00 $132/€123 approx

About this book

Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method. Presenting the advantages and disadvantages of the various protein techniques, Principles and Reactions of Protein Extraction, Purification, and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.


Contents

Extraction of Proteins. Estimation of Proteins. Electrophoretic Analyses of Proteins. Putrifaction of Proteins. Purification of Glycoproteins and Analyses of their Oligosaccharides. Antibodies: Structures, Interactions and Production. AntibodyLabeling, Antibody Detection and Immunochemical Techniques.

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