About this book
Following on from the same author's 1996 volume, this is in part an update, and in part new techniques and material.
From the publisher's announcement:
Whether considering toxicant exposure in zebrafish, or the application of cellular diagnostics to marine toxicology, or the ecotoxicology of coral reef ecosystems, or the amount of metalloids in water, this reference offers the protocols for specimen collection that researchers need. Following up on his popular Techniques in Aquatic Toxicology with a second volume, now nine years later, Dr. Ostrander has once again called on the top aquatic toxicologists from across the world to present 39 chapters of unique collection and testing procedures.
Updating five techniques from the first volume, the authors have gone on to add over two dozen new techniques. Like the first volume, this text divides the chapters into four broad areas: Techniques for the Assessment of Toxicity in Whole Organisms, Cellular and Subcellular Toxicity, Contaminant Identification, and Impacts in Aquatic Ecosystems, and ends with a General Techniques section that anyone working in the field should find useful.
Every chapter covers a specific procedure that can easily be reproduced by any competent technician with basic knowledge. Each of the chapter authors provides and interprets typical as well as anomalous results, false positives, and artifacts. Data is provided either from recently published experiments or from work being published for the first time.
Contents
Techniques for assessment of toxicity in whole organismsIntegrative measures of toxicant exposure in zebrafish (Danio rerio) at different levels of biological organization. Roel Smolders, Wim De Coen, and Ronny BlustUse of disease challenge assay to assess immunotoxicity of xenobiotics in fish. Mary R. Arkoosh, Deborah Boylen, Coral L. Stafford, Lyndal L. Johnson, and Tracy K. CollierEnhanced frog embryo teratogenesis assay - Xenopus (FETAX) model using Xenopus tropicalis.Douglas J. Fort and Robert L. RogersA short-term mummichog (Fundulus heteroclitus) bioassay to assess endocrine responses to hormone-active compounds and mixtures. Deborah L. MacLatchy, Karen L. Gormley, Rebecca E. M. Ibey, Rainie L. Sharpe, Kevin S. Shaughnessy, Simon C. Courtenay, MoniqueG. Dub?, and Glen J. Van Der KraakConducting dose-response feeding studies with salmonids: growth as an endpoint. James P. Meador, Frank C. Sommers, Robert J. Wolotira, and Leslie KubinField experiments with caged bivalves to assess chronic exposure and toxicity. Michael H. Salazar and Sandra M. SalazarApplication of computer microscopy for histopathology in isopod toxicity studies. Damjana Drobne and Samo DrobneSperm cell and embryo toxicity tests using the sea urchin Paracentrotus lividus (LmK). Annamaria Volpi Ghirardini, Alessandra Arizzi Novelli, Chiara Losso, and Pier Francesco GhettiAssessment of metal toxicity to sulfate reducing bacteria through metal concentration methods. Vivek P. Utgikar, and Henry H. TabakTechniques for measurement of cellular and subcellular toxicityCellular diagnostics and its application to aquatic and marine toxicology. Craig A. DownsA non-destructive technique to measure cytochrome P4501A enzyme activity in living embryos of the estuarine fish Fundulus heteroclitus. Diane Nacci, Laura Coiro, Deena M. Wassenberg, and Richard T. Di GiulioDetermination of lipid classes and lipid content in tissues of aquatic organisms using a thin layer chromatography/flame ionization detection (TLC/FID) microlipid method. Gina M. Ylitalo, Gladys K. Yanagida, Lawrence Hufnagle, Jr., and Margaret M. KrahnLarval molting hormone synthesis and imaginal disc development in the midge Chironomus riparius as tools for assessing the endocrine modulating potential of chemicals in aquatic insects. Torsten HahnThe electro-olfactogram: an in vivo measure of peripheral olfactory function and sublethal neurotoxicity in fish. David H. Baldwin and Nathaniel L. ScholzEnzyme-linked immunosorbent assay for screening estrogen receptor binding activityTomoko Koda, Yoshihiro Soya, and Masatoshi MoritaLysosomal destabilization assays for estuarine organisms. A. H. Ringwood, D. E. Conners, J. Hoguet, and L. A. RingwoodIMCOMP-P: an assay for coral immuno-competence. Craig A. Downs, Aaron G. Downs, Robert B. Jonas, Kay Briggs, Thomas Capo, and Cheryl M. WoodleyMonitoring gene expression in Rana catesbeiana tadpoles using a tail f
Customer Reviews