Biological Aging Methods and Protocols

About this book

The goal of Biological Aging: Methods and Protocols is to present some of the most promising and important tools that are currently used in biological aging research. These tools include established protocols such as aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies and the use of ribozymes in aging research. Collectively, these powerful tools combined with the many other techniques that are presented are rapidly advancing the exciting and expanding field of biological aging.

Contents

Contents:; Preface; Contributors; Techniques for Analysis of Biological Aging; Trygve O. Tollefsbol; Aging Cell Culture: Methods and Observations; Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol; Methods to Detect Biomarkers of Cellular Senescence: The Senescence Associated [beta]-galactosidase; Koji Itahana, Judith Campisi, and Goberdhan P. Dimri; Methods of Cell Sorting of Young and Senescent Cells; Joao F. Passos and Thomas von Zglinicki; Telomere Length Analysis; Andres Canela, Peter Klatt, and Maria A. Blasco; A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems; Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol; A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro; Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol; The Chronological Life Span of Saccharomyces Cerevisiae; Paola Fabrizio and Valter D. Longo; Caloric Restriction and Life-Span Determination of Yeast Cells; Oliver Medvedik and David A. Sinclair; Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy or Beef; Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Ruden; Caloric Restriction Mimetics: A Novel Approach for Biogerontology; Mark A. Lane, George S. Roth, and Donald K. Ingram. Extension of Cell Life-Span Using Exogenous Telomerase; Mo K. Kang and No-Hee Park; Inducing Cellular Senescence Using Defined Genetic Elements; Hiroshi Nakagawa and Oliver G. Optiz; Methods of Induction of Cellular Senescence Using Oxidative Stress; Jian-Hua Chen, Susan E. Ozanne, and C. Nicholas Hales; Nuclear Transfer Methods to Study Aging; Lin Liu and David L. Keefe; Use of Ribozymes in Cellular Aging Research; Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa; Methods of Evaluation of Pharmacological Drugs Effects on Aging and Life Span in Mice; Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski; Identifying Genes that Extend Life-Span Using a High Throughput Screening System; Cuiying Chen and Roland Contreras; Application of DNA Microarray Technology to Gerontological Studies; Sergey V. Anisimov; Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus Musculus and Drosophila Melanogaster; Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg; Subtractive Hybridization Techniques to Study Cellular Senescence; Michal B. Sternberg and Shimon Gepstein; Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity; Maria De Luca and Jeff Leips; Quantitative Trait Locus (QTL) Mapping in Aging Systems; Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz.

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