Part I. General; Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations; Alec W. M. Simpson; Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2; Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert; Measurement of [Ca2+]i in Cell Suspensions Using Indo-1; Adriaan Nelemans; Part II. Specialist Measurement Systems; Confocal Microscopy: Theory and Applications for Cellular Signaling; Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars; Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i); Anthony J. Morgan and Andrew P. Thomas; Ratiometric Ca2+ Measurements Using the FlexStation[registered] Scanning Fluorometer; Ian C. B. Marshall, Izzy Boyfield, and Shaun McNulty; Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR[registered]); Ian C. B. Marshall, Davina E. Owen, and Shaun McNulty; Part III. Measurement of Ca 2+ Channel Activity; Measurement of Ca2+ Entry into Cells Using 45Ca 2+; Mercedes Villarroya, Manuela G. Lopez, Maria F. Cano-Abad, and Antonio G. Garcia; Measurement of [3H]PN200-110 and w-Conotoxin MVIIA Binding; Kazuyoshi Hirota and David G. Lambert; Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca 2+ Channel Currents in Single Cells: Heterologous Expression Systems and Dissociated Brain Neurons; Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani; Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores; Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells; Alison Skippen, Philip Swigart, and Shamshad Cockcroft.; Measurement of Inositol(1,4,5)triphosphate Using a Stereospecific Radioreceptor Mass Assay; Darren Smart; Measurement of Calcium Fluxes in Permeabilised Cells Using a 45Ca 2+ Uptake and Release Assay; Robert A. Wilcox; Microinjection of myo-inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells; Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann; Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators; Aldebaran M. Hofer. Part V. Specialist Measurement Techniques; Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry; Hideo Kanaide; Measurement of Calcium and Movement in Heart Cells; Leong L. Ng and Paulene A. Quinn; Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations; Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch; Measurement of Cytosolic-Free Ca2+ in Plant Tissue; Martin R. McAinsh and Carl K.-Y Ng; Part VI. Ca2+ Sensitive Targets; Assay and Purification of Calmodulin-Dependent Protein Kinase; Rajendra K. Sharma; Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation; Rajendra K. Sharma; Measurement of Ca2+-ATPase activity (in PMCA and SERCA1); Danuta Kosk-Kosicka.