A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Human ES Cell; Methods and Protocols; Kursad Turksen; Human ES cells and differentiation; Ariff Bongso; Blastocyst Culture For Deriving Human Embryonic Stem Cells; Woo Suk Hwang, Byeong Chun Lee and Sung Keun Kang; Establishment of a Pluripotent human embryonic stem cell line from a cloned blastocyst.; Mark Richards and Ariff Bongso; Propagation of Human Embryonic Stem Cells on Human Feeder Cells; Michal Amit and Joseph Itskovitz-Eldor; Derivation and maintenance of human embryonic stem cells; Maisam Mitalipova and Gail Palmarini; Isolation and characterization of human ES cells; Jim McWhir Davina Wojtacha & Alison Thomson; Routine culture and differentiation of human ES cells; Michal Amit and Joseph Itskovitz-Eldor; Maintenance of human embryonic stem cells in animal-serum- and feeder-layer-free culture conditions cells on human feeder layers.; Ali H. Brivanlou; Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor. Chui-Yee Fong and Ariff Bongso; Derivation of Human Feeders For Prolonged Support of Human Embryonic Stem Cells; Ronald S. Goldstein; Transplantation of human embryonic stem cells to the chick embryo.; Melissa Carpenter, Mahendra S. Rao, William J. Freed and Xianmin Zeng.; Neurons from hES cells in vitro.; Xue-Jun Li and Su-Chun Zhang; In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells.; Lisheng Wang, Chantal Cerdan, Pablo Menendez and Mickie Bhatia; Derivation and characterization of hematopoietic cells from human embryonic stem cells.; Pablo Menendez, Lisheng Wang, Chantal Cerdan, and Mickie Bhatia; Retroviral transduction of hematopoietic progenitors derived from hESC.; Rachel Eiges; Genetic manipulation of human embryonic stem cells by transfection; Yongquan Luo, Bhaskar Bhattacharya, Amy X. Yang, Raj K. Puri and Mahendra Rao.; Designing, testing and validating microarray for characterization of stem cells.; Maria Wartenberg, Andreas Finkensieper, Jurgen Hescheler and Heinrich Sauer.; Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis.; Maty Tzukerman, and Karl Skorecki; A novel experimental platform for investigating cancer growth and anti-cancer therapy in a human tissue micro-environment derived from human embryonic stem cells; Justin C. St. John, Alexandra Amaral, Emma Bowles, Joao Facucho, Rhiannon Lloyd, Mariana Freitas, Heather L. Gray, Christopher S. Navara, Gisela Oliveira, Gerald P. Schatten, Emma Thomas and Joao Ramalho-Santos; The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells.
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