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Microchip Capillary Electrophoresis: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 339

Edited By: Charles Henry

Humana Press

Hardback | Dec 2006 | #163660 | ISBN: 1588292932
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £108.00 $132/€121 approx

About this book

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.


Part I. Microchip Fabrication Methods Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel, Jr., Richard P. Baldwin, John F. Naber, and Douglas J. Jackson Micro-Molding for Poly(dimethylsiloxane) Microchips Carlos D. Garcia and Charles S. Henry Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation Laurie E. Locascio, David J. Ross, Peter B. Howell, and Michael Gaitan Part II. Surface Modification Methods Surface Modification Methods for Enhanced Device Efficacy and Function Barbara J. Jones and Mark A. Hayes Polyelectrolyte Coatings for Microchip Capillary Electrophoresis Yan Liu and Charles S. Henry Part III. Detection Methods for Microchip Capillary Electrophoresis Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry Trust Razunguzwa and Aaron T. Timperman Interfacing Amperometric Detection With Microchip Capillary Electrophoresis R. Scott Martin Conductivity Detection on Microchips Roland Hergenroder and Benedikt Grass Part IV. Applications of Microchip Capillary Electrophoresis DNA Separations Andrea W. Chow Protein Separations Andrea W. Chow Microchip Capillary Electrophoresis: Application to Peptide Analysis Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte Measuring Electroosmotic Flow in Microchips and Capillaries S. Douglass Gilman and Peter J. Chapman Single Cell Analysis on Microfluidic Devices Christopher T. Culbertson Rapid DNA Amplification in Glass Microdevices Christopher J. Easley, Lindsay A. Legendre, James P. Landers, and Jerome P. Ferrance Index

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