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Handbook of Cryopreparation Methods for Electron Microscopy

Handbook / Manual

Edited By: Annie Cavalier, Daniele Spehner and Bruno Humbel

CRC Press

Hardback | Mar 2008 | #171291 | ISBN-13: 9780849372278
Availability: Usually dispatched within 6 days Details
NHBS Price: £114.00 $145/€134 approx

About this book

There has been exponential growth in cryo-electron microscopy methods over the last twenty years. Using an accessible, consistent format to explain the material, Handbook of Cryopreparation Methods for Electron Microscopy presents the most sophisticated methods used in research laboratories. It describes the basic principles of freezing, cryo-ultramicrotomy, embedding at low temperatures and freeze-fracture. The book also explores applications such as electron crystallography, 2D/3D structure, tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols and numerous illustrations to clarify the techniques.


Contents

Cryo-Fixation Methods. Macromolecular Assemblies. Cryo-ultramicrotomy. Low-Temperature Embedding. Freeze-Fracture and Metal Shadowing. Immunolabeling, Electron Tomography.

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Biography

University de Rennes, Rennes, Cedex, France University of Strasbourg, Illkirch, France Utrecht University, Netherlands

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