Microarrays and Microplates Applications in Biomedical Sciences

About this book

This title looks at the new microarray and microplate-based technologies which facilitate large-scale analysis of DNA sequence variants, mRNA molecules and proteins. The book provides a review of the various methodologies being used to identify genetic variants and gene regulation and guides readers through both the application of these methodologies and experimental procedures.

Contents

Ch 1. INSTRUMENTATION FOR GENOMICS AND PROTEOMICS, S.K. Stephens, M. Truesdale and J.F. Burke. Introduction. History of the microplate. Microplate-handling instrumentation. Applications in genomics. Applications in proteomics. Conclusion. Ch 2. EXPRESSION MICROARRAYS, C. Whatling, S. Ye and P. Eriksson. Introduction. Microarrays in overview. Array fabrication. Preparation of labelled probes. Microarray hybridization. Generation and analysis of expression data. The need for databases. Validation of gene expression changes. Summary and future prospects. Ch 3. TISSUE MICROARRAYS: A HIGH-THROUGHPUT TECHNOLOGY FOR TRANSLATIONAL RESEARCH, S. Henshall. Introduction. Tissue microarray construction. Staining of tissue microarrays. Tissue microarray data and image management. Applications in translational research. Validation of tissue microarrays. Future developments. Ch 4. PROTEIN CHIPS AND MICROARRAYS, C.D. O'Connor K. Pickard. Introduction. Microarray technology. Versatility of protein microchips. Concluding remarks. Ch 5. SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING USING MICROARRAY, T. Pastinen. Introduction. DNA microarrays for sequence analysis. Arrays for resequencing and SNP-scoring. Summary and future directions. Ch 6. MICROPLATE-BASED mRNA ANALYSIS, T. Murakami and M. Mitsuhashi. Introduction. Microplate-based RNA purification. Microplate-based PCR. Microplate-based non-RT-PCR assays. Application of mRNA analysis to drug discovery project. Application of mRNA analysis to clinical diagnostics. Ch 7. A GENERALIZED 96-WELL ELISA-BASED ASSAY FOR QUANTITATIVE AND QUALITATIVE MONITORING OF CELLULAR EVENTS, L. Shumway and L. M. Schwartz. Introduction. The 96-well in situ ELISA assay. Technical considerations. Suggested statistical treatment. Additional applications of the assay. Ch 8. ELECTROPHORESIS IN MICROPLATE FORMATS, I.N.M. Day, M.A. Al-Dahmesh, K.K. Alharbi, X. Chen, R.H. Ganderton, T.R. Gaunt, L.J. Hinks, S.D. O'Dell, E. Spanakis, P.J.R. Day, M.A. Suchard, B.B. Zhang and M.R. James. Introduction. MADGE, 96-well formats. 192-well, 384-well, 768-well MADGE, ARMS and SNP typing. 96-well high resolution MADGE. Temporal thermal ramp MADGE electrophoresis (Melt-MADGE). Software applications (applies to all MADGE approaches). The future

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