Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses.
The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.
From the reviews of the second edition: "This book describes a wide range of techniques on the molecular analyses of plant viruses and some of their interactions with their hosts. Many techniques could also be of interest to scientists studying animal and bacterial viruses. ! this book should be of great use to virology researchers who take the correct approach." (Roger Hull, Microbiology Today, February, 2009)
Section 1 General Introduction 1. Plant virus interactions --Peter Palukaitis, John Carr and James Schoelz (EJ) 2. Role of capsid proteins -- John Bol (EJ) 3. Role of plant virus movement proteins -- Michael Taliansky, Lesley Torrance, Natalia O. Kalinina (EJ) 4. Multiple roles of viral replication proteins in plant RNA virus replication-- Peter Nagy and Judith Pogany (PN) 5. Role of silencing suppressor proteins --Jozsef Burgyan (EJ) 6. Role of vector transmission proteins -- Veronique Ziegler Graff, Veronique Brault (EJ) Section 2 Viral nucleic acid sequence/function analysis 7. In vivo analysis of Viral RNA translation- William Staplin, Allen Miller (PN) 8. In vitro analysis of translational enhancer --Aurelie Rakotondrafara, Allen Miller (YH) 9. Identification of plant virus IRES -- Sek-Man Wong, Dora Chin-Yen Koh, DingXiang Liu (YH) 10. Analysis of geminivirus DNA replication by 2-D gel - Keith Saunders (YH) 11. Begomoviruses: Molecular cloning and identification of replication origin - Lilian H. Florentino, Anesia A. Santos, Francisco M. Zerbini, Elizabeth P. B. Fontes (YH) 12. Analysis of viroid replication - Ricardo Flores, Maria-Eugenia Gas, Diego Molina, Carmen Hernandez, and Jose-Antonio Daros (PN) 13. Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs- Young-Chan Kim and C. Cheng Kao (PN) 14. In situ detection of plant viruses and virus-specific products - Andy Maule and Zoltan Havelda (PN) 15. Detection of siRNAs and miRNAs- Sakari Kauppinen, Zoltan Havelda (EJ) 16. Cloning of short interfering RNAs from virus infected plants - Thien X. Ho, Rachel Rusholme, Tamas Dalmay, and Hui Wang (YH) 17. Solution structure probing of RNA structures -- Marc R. Fabian and K. Andrew White (PN) 18. RNA encapsidation assay - Padmanaban Annamalai and A. L. N. Rao (GF) Section 3 Protein analysis and investigation of protein function 19. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus- KS. Rajendran and Peter Nagy (GF) 20. Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis- Raul C. Gomila and Lee Gehrke (PN) 21. Probing interactions between plant virus movement proteins and nucleic acids - Tzvi Tzfira, Vitaly Citovski (PN) 22. Movement profiles, a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins - Kateryna Trutnyeva, Pia Ruggenthaler, Elisabeth Waigmann (PN) 23. Analysis of siRNA-supressor of gene silencing interactions - Lorant Lakatos and Jozsef Burgyan (EJ) 24. Phosphorylation analysis of plant virus proteins - Kristiina M. Makinen and Konstantin I. Ivanov (PN) 25. Analysis of interactions between viral replicase proteins and plant intracellular membranes- Helene Sanfacon and Guangzhi Zhang (PN) 26. Membrane and protein dynamics in virus-infected plant cells - Michael Goodin, Romit Chakrabarty, Sharon Yelton (PN) 27. Site-directed mutagenesis of whole viral genomes - Li liu, George Lomonossoff (YH) 28. Viral protein-nucleic acid interaction: South(North)-Western blot - Huanting Liu (YH) 29. Protein-protein interactions: yeast two hybrid system - Deyin Guo, Minna-Liisa Rajamaki, and Jari Valkonen (YH) 30. NMR analysis of viral protein structures - Andrew J. Dingley, Inken Lorenzen and Joachim Grotzinger (YH) 31. Localization of viral protein in plant cells: protein tagging --Sophie Haupt, Lesley Torrance (GF) Section 4 Microscopy/GFP/Protein tagging/infections clones and other such tools 32. Construction of infectious clones for RNA viruses - TMV # Sean Chapman (YH) 33. Construction of infectious clones for RNA viruses -TCV # Eugene Ryabov (YH) 34. Construction of infectious clones for DNA viruses -Mastrevirus -Margaret Boulton (EJ) 35. Construction of infectious clones of double-stranded DNA viruses of plants using Citrus Yellow Mosaic Virus as an example- Qi Huang, John S. Hartung (EJ) 36. Insertion of Introns -- A Strategy to Facilitate Assembly of Infectious Full Length Clones-Elisabeth Johansen and Ole Sogaard Lund (GF) 37. Analysis of cell-to-cell and long-distance movement of Apple latent spherical virus in infected plants using green, cyan, and yellow fluorescent proteins -- Tsubasa Takahashi, Nobuyuki Yoshikawa (GF) 38. Agroinoculation: A simple procedure for systemic infection of plants with virus -- Zarir Vaghchhipawala, Kirankumar Mysore (GF) 39. Geminvirus biolistic inoculation and molecular diagnosis -- Anesia A. Santos, Lilian H. Florentino, Acassia M. B. Leal, Elizabeth P. B. Fontes (YH) Section 5 Genomics, Host factors and plant based studies 40. Expression microarrays in plant-virus interactions- Gruden K., Pompe-Novak M., Baebler S., Krecic-Stres H., Toplak N., Hren M., Kogov sek, P., Gow L., Foster G., Boonham N., Ravnikar M. (GF) 41. Genome-wide screens for identification of host factors in viral replication- Tadas Panavas, Elena Serviene, Judit Pogany and Peter D. Nagy (PN) 42. Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase- Jung Younlee (GF) 43. Virus induced gene silencing as a tool to identify host genes affecting viral pathogenicity - Xiaohong Zhu and Dinesh-Kumar, S.P (GF) 44. Yeast Two-hybrid assay to identify host-virus interactions- Stuart A. MacFarlane and Joachim F. Uhrig (EJ)
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