To see accurate pricing, please choose your delivery country.
 
 
United States
£ GBP
All Shops

British Wildlife

8 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £33 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £26 per year
Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Technical Aspects and Principles of PCR Amplification

By: Elizabeth van Pelt-Verkuil, Alexander van Belkum and John P Hays
340 pages
Technical Aspects and Principles of PCR Amplification
Click to have a closer look
  • Technical Aspects and Principles of PCR Amplification ISBN: 9781402062407 Hardback Mar 2008 Not in stock: Usually dispatched within 1-2 weeks
    £129.99
    #174012
Price: £129.99
About this book Contents Customer reviews Related titles

About this book

Kary Mullis was awarded a Nobel prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical diagnostic and research laboratories across the world, from academic laboratories purely interested in basic research to diagnostic laboratories using high throughput routine PCR testing methodologies to detect (for example) minimum residual disease and the presence/absence of particular pathogens.

Frequently, PCR technicians limit their understanding of PCR to that particular methodology they are currently familiar with. However, this approach limits their understanding and appreciation of the range of versatile PCR techniques currently available. Techniques which may be applicable, and indeed more suitable, to their own laboratory situation. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
As such, many important technical issues have been included in the book, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control and quality assurance, variants and adaptations of the standard PCR protocol, quantitative PCR and in situ PCR.

Contents

Chapter 1. The Polymerase Chain Reaction Chapter 2. A Brief Comparison Between In Vivo DNA Replication And In Vitro PCR Amplification Chapter 3. The PCR In Practice Chapter 4. The Different Types And Varieties Of Nucleic Acid Target Molecules Chapter 5: PCR Primers Chapter 6. Deoxynucleotide Triphosphates And Buffer Components Chapter 7. Taq And Other Thermostable DNA Polymerases Chapter 8. Important Considerations For Typical, Quantitative And Real-Time PCR Protocols Chapter 9. Analysis Of PCR Amplification Products. Chapter 10. Ensuring PCR Quality - Laboratory Organisation, Pcr Optimisation And Controls. Chapter 11. Ensuring PCR Quality-Quality Criteria And Quality Assurance. Chapter 12. Variants And Adaptations Of The Standard Pcr Protocol Chapter 13. In Situ PCR Amplification (Isa) - Major Considerations, Sample Processing And Applications

Customer Reviews

By: Elizabeth van Pelt-Verkuil, Alexander van Belkum and John P Hays
340 pages
Current promotions
New and Forthcoming BooksNHBS Moth TrapBritish Wildlife MagazineBuyers Guides