In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In this book, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics.
From the reviews of the second edition: "In this second edition it is particularly stressed the cytoskeleton study at the different levels of life organization ! . chapters incorporate a clear introduction, a useful lists of the necessary materials and reagents and the usual (so appreciated by beginners) step-by-step laboratory protocols to avoid the troubleshooting and pitfalls of the bench journey. Cell and developmental biologists are the primary targets as an audience ! ." (CarloAlberto Redi, European Journal of Histochemistry, Spring, 2010) "The book contains an interesting mix of chapters some of which cover basic concepts and ideas that are easily transferrable to other systems and others that are more specialised. ! overall the book contains many useful nuggets of information and will be very useful for anyone wanting to try out a new technique or approach covered by the various chapters." (Michelle Peckham, Infocus Magazine, Issue 21, March, 2011)
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