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Imaging in Developmental Biology: A Laboratory Manual

Handbook / Manual

Series: Imaging

Edited By: James Sharpe and Rachel O Wong

883 pages, 168 colour & 37 b/w illustrations

Cold Spring Harbor Laboratory Press

Paperback | Dec 2010 | #194562 | ISBN-13: 9780879699406
Availability: Usually dispatched within 6 days Details
NHBS Price: £133.00 $163/€149 approx
Hardback | Dec 2010 | #194564 | ISBN-13: 9780879699390
Availability: Usually dispatched within 6 days Details
NHBS Price: £193.00 $236/€217 approx

About this book

New imaging technologies have revolutionized the study of developmental biology. Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process.

"Imaging in Developmental Biology: A Laboratory Manual", a new volume in Cold Spring Harbor Laboratory Press Imaging series, presents a comprehensive set of essential visualization methods. The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. Methods range from the basics of labeling cells to cutting-edge protocols for high-speed imaging, optical projection tomography, and digital scanned laser light-sheet fluorescence. Imaging has become a required methodology for developmental biologists, and Imaging in Developmental Biology: A Laboratory Manual provides the detailed explanations and instructions for mastering these necessary techniques.


Preface to the Book Series
1. Introduction

2. Live Imaging of Caenorhabditis elegans Embryogenesis
3. Live Imaging of Drosophila Development
4. Live Imaging of Zebrafish Development
5. Live Imaging of Cells and Tissues in Xenopus Embryos
6. Live Imaging of Avian Embryos
7. Live Imaging of Mouse Embryos


Labeling Cells for Live Cell Imaging
8. Electroporation of Chick and Mouse Embryos
9. Single-Cell Electroporation in Xenopus
10. Single-Neuron Labeling Using Genetic Methods
11. Ballistic Labeling of Developing Retinal Neurons
12. Multicolor Brainbow Imaging in Zebrafish
13. Genetic Labeling of Neurons in Mouse Brain

Cell Fate, Differentiation, and Migration
14. Live Imaging of Neuroblast Lineages within Intact Larval Brains in Drosophila
15. Visualizing Cell Contacts and Cell Polarity in Caenorhabditis elegans Embryos
16. In Vivo Birthdating in Zebrafish
17. Imaging Retinal Progenitor Lineages in the Developing Zebrafish Retina
18. Imaging the Development of the Fish Lateral-Line System
19. Imaging Cell Migration in Chick Explant Cultures
20. Imaging Cell Movements in Egg Cylinder Stage Mouse Embryos
21. Marking Cells for Imaging Analysis of Morphogenetic Behavior and Cell Fates in Mouse Embryos
22. Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progressions
23. Time-Lapse Imaging of Fluorescently Labeled Live Cells in the Embryonic Mammalian Forebrain

Imaging Circuit Formation in the Nervous System
24. Imaging Calcium Transients in Developing Xenopus Spinal Neurons
25. Imaging Axon Pathfinding in Xenopus and Zebrafish In Vivo
26. Axons and Dendrites: In Vivo Time-Lapse Imaging of Neuronal Development in Xenopus
27. In Vivo Imaging of Synaptogenesis in Zebrafish
28. Live Imaging of Developing Retinal Circuits
29. Imaging the Developing Mammalian Neuromuscular Junction In Vivo
30. Live Imaging of Developing Hippocampal Neurons in Culture
31. Imaging of Synapse Formation and Function in Neuronal Cell Cultures by Quantitative Immunocytochemistry
32. Simultaneous Imaging of Structural Plasticity and Calcium Dynamics in Developing Dendrites and Axons
33. Simultaneous Patch-Clamping and Calcium Imaging in Developing Dendrites
34. Imaging Synaptic Protein Dynamics in the Cerebral Cortex In Vitro and In Vivo
35. In Vivo Imaging of Axonal and Dendritic Structures in Developing Cortex

36. Quantifying the Bicoid Morphogen Gradient in Living Fly Embryos
37. Quantitative Imaging of Morphogen Gradients in Drosophila Imaginal Discs
38. Methods to Investigate Molecular Mechanisms and Cellular Mechanics Responsible for Morphogenesis in Xenopus laevis Embryos
39. Notochord Formation in Zebrafish
40. High-Resolution, Multiphoton Time-Lapse Imaging of Early Chick Embryos
41. Four-Dimensional Fluorescent Imaging of Embryonic Quail Development
42. Imaging Kidney Development
43. Practical Considerations for Long-Term Time-Lapse Imaging of Epithelial Morphogenesis in Three-Dimensional Organotypic Cultures
44. High-Speed Optical Coherence Tomography Imaging of the Beating Avian Embryonic Heart
45. Imaging Mouse Embryonic Cardiovascular Development
46. Micro-Ultrasound and Its Application to Longitudinal Studies of Mouse Eye Development and Disease

47. Quantitative Imaging of Gene Expression in Drosophila Embryos
48. Three-Dimensional Morphology and Gene Expression Mapping for the Drosophila Blastoderm
49. From Confocal Imaging to 3D Model: A Protocol for Creating 3D Digital Replicas of Ascidian Embryos
50. Preparation of Fixed Xenopus Embryos for Confocal Imaging
51. Optical Projection Tomography of Vertebrate Embryo Development
52. X-Ray Microtomographic Imaging of Vertebrate Embryos
53. Episcopic Three-Dimensional Imaging of EmbryosTimothy J. Mohun and Wolfgang J. Weninger
54. High-Throughput Analysis of Mouse Embryos by Magnetic Resonance Imaging
55. MRI in Developmental Biology and the Construction of Developmental Atlases
56. Automated Lineage and Expression Profiling in Live Caenorhabditis elegans Embryos
57. Imaging the Development of Entire Zebrafish and Drosophila Embryos with Digital Scanned Laser Light-Sheet Fluorescence Microscopy (DSLM)

1. Electromagnetic Spectrum
2. Fluorescence Microscopy Filters and Excitation/Emission Spectra
3. Safe Operation of a Fluorescence Microscope
4. Microscope Objective Lenses
5. Resources for Live Imaging of Drosophila
6. Glossary of Imaging Terms
7. Cautions

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