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Drosophila Neurobiology: A Laboratory Manual

Handbook / Manual

Edited By: Bing Zhang, Marc R Freeman and Scott Waddell

534 pages, 70 colour & 43 b/w illustrations

Cold Spring Harbor Laboratory Press

Paperback | Jul 2010 | #197272 | ISBN-13: 9780879699055
Availability: Usually dispatched within 6 days Details
NHBS Price: £121.00 $154/€144 approx

About this book

Cold Spring Harbor Laboratory's long-running Neurobiology of Drosophila course has trained a generation of neuroscientists, many of whom have become leaders in the field. "Drosophila Neurobiology: A Laboratory Manual" offers the detailed protocols and background material developed by the course instructors to all researchers interested in using Drosophila as an experimental model for investigating the nervous system. The manual covers three approaches to the field: Studying Neural Development in Drosophila melanogaster, Recording and Imaging in the Drosophila Nervous System, and Studying Behavior in Drosophila. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods. Written by leading experts from the community, "Drosophila Neurobiology: A Laboratory Manual" is an essential guide for researchers at all levels, from the beginning graduate student through the established primary investigator.

This almost 'foolproof' manual is a 'must have' for every Drosophila neurobiology laboratory, and is a tribute to the creativity of the fly community for steadily advancing technologies to label, manipulate, or examine neurons....Each chapter is well organized, and features a concise introduction, critical references, and detailed experimental protocols that cover basic and cutting-edge techniques, many of which have revolutionized the way we do research....Positively, this specialized manual will be an unparalleled resource for the fly neurobiology novice and aficionado.
- The Quarterly Review of Biology


Contents

SECTION 1: STUDYING NEURAL DEVELOPMENT IN DROSOPHILA MELANOGASTER
1. Studying Neural Development in Drosophila melanogaster: An Introduction to Section 1
2. Molecular and Cellular Analyses of Larval Brain Neuroblasts in Drosophila melanogaster
3. Cell-Fate Determination in the Embryonic Central Nervous System
4. Analysis of Axon Guidance in the Embryonic Central Nervous System of Drosophila
5. Analysis of Glial Cell Development and Function in Drosophila
6. Methods for Exploring the Genetic Control of Sensory Neuron Dendrite Morphogenesis
7. Embryonic and Larval Neuromuscular Junction: An Overview with Selected Methods and Protocols
8. Genetic Mosaic Analysis of Drosophila Brain by MARCM
9. Studying Olfactory Development and Neuroanatomy with Clonal Analysis
10. Dissecting and Staining Drosophila Optic Lobes

SECTION 2: RECORDING AND IMAGING ACTIVITIES IN THE DROSOPHILA NERVOUS SYSTEM
11. Recording and Imaging in the Drosophila Nervous System: An Introduction to Section 2
12. Synaptic Physiology at the Drosophila Neuromuscular Junction
13. Electrophysiological Recordings from the Drosophila Giant Fiber System
14. Analysis of Visual Physiology in the Adult Drosophila Eye
15. Measuring Sound-Evoked Potentials from Drosophila Johnston's Organ
16. Chemosensory Coding in Single Sensilla
17. Electrophysiological Recording from Neurons in Drosophila Embryos and Larvae
18. In Vivo Whole-Cell Recordings in the Drosophila Brain
19. Calcium Imaging
20. Imaging Neuropeptide Release and Signaling in the Drosophila Neuromuscular Junction with Green Fluorescent Protein
21. In Vivo Imaging of Drosophila Larval Neuromuscular Junctions to Study Synapse Assembly
22. Experimental Methods for Examining Synaptic Plasticity
23. Acute Inactivation of Proteins Using FIAsH-FALI

SECTION 3: STUDYING BEHAVIOR IN DROSOPHILA
24. Studying Behavior in Drosophila: An Introduction to Section 3
25. Visual Learning and Perception in Drosophila
26. Aversive and Appetitive Olfactory Conditioning
27. Odor-Taste Learning in Larval Drosophila
28. Studying Aggression in Drosophila
29. Courtship
30. Measurement of Courtship Plasticity in Drosophila
31. Sleep and Circadian Behavior Monitoring in Adult Drosophila
32. Feeding Behavior of Drosophila Larvae

Appendix: Cautions
Index

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