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Immunocytochemical Methods and Protocols

Handbook / ManualOut of Print

Series: Methods in Molecular Biology Volume: 34

Edited By: LC Javois


Paperback | Dec 1999 | Edition: 2 | #95694 | ISBN: 0896035700
Out of Print Details
Hardback | Dec 1999 | Edition: 2 | #95695 | ISBN: 0896038130
Out of Print Details

About this book

Spiral-bound edition for benchtop use.

This second edition provides detailed protocols for immunocytochemical localization of tissue constituents. The book is well balanced and suited for basic and clinical research scientists...The editor has successfully compiled this book for the intended readers. The editor and contributors are credible...The protocol style format is useful for a first experiment. The illustrations are attractive and informative. The book as a whole is nicely done...I find this a very useful and practical addition to all fields of biology and medicine. It is very user friendly and should be a great reference for those designing experiments and writing. - Doody's Health Sciences Book Review Journal ...this book would make a valuable addition to laboratories currently involved in research using immunocytochemical techniques and would be particularly useful for those wishing to introduce these techniques into their research programmes...Given the depth of coverage of theory behind various techniques and the detailed notes that accompany each set of protocols, the book would also make an ideal teaching aid. - Molecular Biotechnology this excellent book on Methods and Protocols can be recommended to both investigators starting out in the field of immunocytochemistry and to basic and clinical researchers already working in the field because it contains a plethora of methods and protocols for the demonstration of molecules in many different normal and pathological situations and also under different experimental conditions. - Journal of Chemical Neuroanatomy


Part I.Antibody Preparation. Overview of Antibody Use in Immunocytochemistry Su-Yau Mao Lorette C. Javois and Ute M. Kent. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration Ute M. Kent. Purification of Antibodies Using Ion-Exchange Chromatography Ute M. Kent. Purification of Antibodies Using Affinity Chromatography Ute M. Kent. Purification of Antibodies Using Protein A-Sepharose and FPLC Ute M. Kent. Conjugation of Fluorochromes to Antibodies Su-Yau Mao. Biotinylation of Antibodies Su-Yau Mao. Part II.Tissue Preparation for light Microscopic Analyses. Overview of Cell Fixatives and Cell Membrane Permeants Melissa A. Melan. Preparation of Frozen Sections for Analysis Gary L. Bratthauer. Processing of Cytological Specimens Gary L. Bratthauer. Processing of Tissue-Culture Cells Gary L. Bratthauer. Processing of Tissue Specimens Gary L. Bratthauer. Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections Laszlo Krenacs Tibor Krenacs and Mark Raffeld. Part III. Light Microscopic Detection Systems. Overview of Fluorochromes J. Michael Mullins. Direct Immunofluorescent Labeling of Cells Lorette C. Javois. Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells Mark C. Willingham. Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells Mark C. Willingham. Fluorescent Labeling of Surface or Intracellular Antigens in Whole- Mounts Lorette C. Javois. TUNEL Assay for Apoptotic Cells Virginia M. Heatwole. Overview of Fluorescence Analysis with the Confocal Microscope Liana Harvath. Overview of Laser Microbeam Applications as Related to Antibody Targeting P. Scott Pine. Overview of Fluorescence Photomicography Lorette C. Javois and J. Michael Mullins. Overview of Antigen Detection Through Enzymatic Activity Gary L. Bratthauer. The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods Gary L. Bratthauer. The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods Gary L. Bratthauer. Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue Mark Raffeld and Elaine S. Jaffe. Multiple Antigen Immunostaining Procedures Laszlo Krenacs Tibor Krenacs and Mark Raffeld. ELISA on Attachment-Dependent or Suspension Grown Cells Julie Ann Brent.. Use of Immunogold with Silver Enhancement Constance Oliver. Part IV. Flourescence Activated Cell Sorter (FACS) Analyses. Overview of Flow Cytometry and Fluorescent Probes for Cytometry Robert E. Cunningham. Tissue Disaggregation Robert E. Cunningham. Indirect Immunofluorescent Labeling of Viable Cells Robert E. Cunningham. Indirect Immunofluorescent Labeling of Fixed Cells Robert E. Cunningham. Fluorescent Labeling of DNA Robert E. Cunningham. Deparaffinization and Processing of Pathologic Material Robert E. Cunningham. Assay for Phagocytosis Liana Harvath and Douglas A. Terle. Assay for Filamentous Actin Liana Harvath. Assay for Chemoattractant Binding Liana Harvath Robert R. Aksamit and Robert E. Cunningham. Assay for Oxidative Metabolism Liana Harvath and Douglas A. Terle. Part V. Colloidal Gold Detection Systems For Electron Microscopic Analysis. Fixation and Embedding Constance Oliver. Preparation of Colloidal Gold Constance Oliver. Conjugation of Colloidal Gold to Proteins Constance Oliver. Colloidal Gold/Streptavidin Methods Constance Oliver. Pre-Embedding Labeling Methods Constance Oliver. Postembedding Labeling Methods Constance Oliver. Part VI.Applications For Nucleic Acid Localization By In Situ Hybridization. Overview of In Situ Hybridization Roland M. Nardone. In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase Labeled Centromeric Probe Roland M. Nardone. Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes Roland M. Nardone. In Situ RT-PCR and Hybridization Techniques Vishakha Thaker. Part VII. Special Applications For the Clinical Laboratory. Overview of the Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines Patricia A. Fetsch and Andrea Abati. Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors Dennis M. Frisman. Overview of Automated Immunostainers Gilbert E. Herman Edna A. Elfont and Alton D. Floyd.

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