Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols.
Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together.
Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
1. The Tomato Leaf as a Model System for Organogenesis Yogev Burko and Naomi Ori
2. Usefulness of Physcomitrella patens for Studying Plant Organogenesis Sandrine Bonhomme, Fabien Nogue, Catherine Rameau, and Didier G. Schaefer
3. The Dicot Root as a Model System for Studying Organogenesis Julie Lavenus, Michael Lucas, Laurent Laplaze, and Soazig Guyomarc'h
4. Genetic Control of Root Organogenesis in Cereals Caroline Marcon, Anja Paschold, and Frank Hochholdinger
5. Gene Expression Analysis of Aquatic Angiosperms Podostemaceae to Gain Insight into the Evolution of Their Enigmatic Morphology Satoshi Koi and Natsu Katayama
6. Brown Algae as a Model for Plant Organogenesis Kenny A. Bogaert, Alok Arun, Susana M. Coelho, and Olivier De Clerck
7. Microscopic Analysis of Ovule Development in Arabidopsis thaliana Balaji Enugutti, Maxi Oelschner, and Kay Schneitz
8. Imaging of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis Cristina Llavata-Peris, Annemarie Lokerse, Barbara Moller, Bert De Rybel, and Dolf Weijers
9. Inducible System for Lateral Roots in Arabidopsis thaliana and Maize Leen Jansen, Boris Parizot, and Tom Beeckman
10. Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root Organogenesis Inge Verstraeten, Tom Beeckman, and Danny Geelen
11. High-Throughput, Non-Invasive Imaging of Root Systems Anjali S. Iyer-Pascuzzi, Paul R. Zurek, and Philip N. Benfey
12. Small Molecule Screens to Study Lateral Root Development Dominique Audenaert, Bert De Rybel, Long Nguyen, and Tom Beeckman
13. Cell Lineage Analyses in Living Tissues John Runions and Smita Kurup
14. Protein Immunolocalization in Maize Tissues Cristian Forestan, Nicola Carraro, and Serena Varotto
15. Auxin Immunolocalization in Plant Tissues Cristian Forestan and Serena Varotto
16. Gene Expression Analysis of Shoot Apical Meristem Cell Types Ram Yishor Yadav, Stephen Snipes, Thomas Girke, and G. Venugopala Reddy
17. Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabuo Fiorani, and Gerrit T.S. Beemster
18. Regeneration in Arabidopsis Tissue Culture Kaoru Sugimoto and Elliot M. Meyerowitz
19. Isolation and Analysis of mRNAs from Specific Cell Types of Plants by Ribosome Immunopurification Angelika Mustroph, Eugenia Zanetti, Thomas Girke, and Julia Bailey-Serres
20. Analyzing Small and Long RNAs in Plant Development Using Non-Radioactive in-situ Hybridization Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Briere, and Martin Crespi
21. Analyzing Protein Distribution in Plant Tissues Using "Whole-Mount" Immunolocalization Pilar Bustons-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Briere, and Martin Crespi
22. Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus Aude Le Bail and Benedicte Charrier
23. Building Simulation Models of Developing Plant Organs Using VirtualLeaf Roeland M.H. Merks and Michael Guravage
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