In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope.
The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology(t) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.
1. A Bird's Eye View of the Bacterial Landscape Natividad Ruiz
2. Visualizing the Bacterial Cell Surface: An Overview Harald Engelhardt
3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems Nadine S. Henderson and David G. Thanassi
4. Experimental Manipulation of the Microbial Functional Amyloid Called Curli Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman
5. Visualization of Gram-Positive Bacterial Pili Chungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That
6. Single Cell Microfluidic Studies of Bacterial Motility Adrien Ducret, Olivier Theodoly, and Tam Mignot
7. Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule Susan M. Steenbergen and Eric R. Vimr
8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy Wei Hu, Renate Lux, and Wenyuan Shi
9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance Elena B. Tikhonova and Helen I. Zgurskaya
10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist
11. Pore Formation by T3SS Translocators: Liposome Leakage Assay Eric Faudry, Caroline Perdu, and Ina Attree
12. Isolation of Bacterial Type IV Machine Subassemblies Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie
13. Production and Crystallization of Bacterial Type V Secretion Proteins Hye-Jeong Yeo
14. Assembly of Bacterial Outer Membrane Proteins Jan Grijpstra, Martine P. Bos, and Jan Tommassen
15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent
16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles Halima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn
17. In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer
18. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes Filipe Carvalho, Maria Graciela Pucciarelli, Francisco Garcia-del Portillo, Didier Cabanes, and Pascale Cossart
19. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis Anna E. Grzegorzewicz and Mary Jackson
20. Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues Katleen Denoncin, Valerie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, and Jean-Francois Collet
21. Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio
22. Isolation of Bacteria Envelope Proteins Shu Quan, Annie Hiniker, Jean-Francois Collet, and James C.A. Bardwell
23. Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels in Giant Spheroplasts of E. coli Boris Martinac, Paul R. Rohde, Charles G. Cranfield, and Takeshi Nomura
24. Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid Bilayers Owen S.Mapingire, Beau Wager, and Anne H. Delcour
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