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Fungal Secondary Metabolism: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 944

By: Nancy P Keller (Editor), Geoffrey Turner (Editor)

300 pages, 32 colour & 28 b/w illustrations, 11 tables

Humana Press

Hardback | Oct 2012 | #201333 | ISBN-13: 9781627031219
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £89.50 $113/€107 approx

About this book

Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field.

Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. It is written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.


Contents

1. Library Preparation and Data Analysis Packages for Rapid Genome Sequencing Kyle R. Pomraning, Kristina M. Smith, Erin L. Bredeweg, Lanelle R. Connolly, Pallavi A. Phatale, and Michael Freitag 2. Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters Natalie D. Fedorova, Venkatesh Moktali, and Marnix H. Medema 3. Media and Growth Conditions for Induction of Secondary Metabolite Production Jens C. Frisvad 4. Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills 5. The "FERMEX" Method for Metabolite-Enriched Fungal Extracts Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills 5. The "FERMEX" Method for Metabolite-Enriched Fungal Extracts Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Ulrike Fohgrub and Frank Kempken Jens C. Frisvad 4. Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills 5. The "FERMEX" Method for Metabolite-Enriched Fungal Extracts Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills 5. The "FERMEX" Method for Metabolite-Enriched Fungal Extracts Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz 6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken James F. Sanchez and Clay C.C. Wang 7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Beate Wollinsky and Shu-Ming Li 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Christiane Wallwey and Shu-Ming Li 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz 10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss 17. Targeted Proteomics for Metabolic Pathway Optimization Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold 18. Investigation of In Vivo Protein Interactions in Aspergillus Spores Kwang Cheol Jeong and Jae-Hyuk Yu 19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus Anindya Chanda, Ludmila V. Roze, and John E. Linz 20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson 21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects Ulrike Fohgrub and Frank Kempken 11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis Jin Woo Bok and Nancy P. Keller 12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii 13. Multi-Copy Genetic Screen in Aspergillus nidulans Hee Soo Park and Jae-Hyuk Yu 14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification Ozgur Bayram, Ozlem Sarikaya Bayram, Oliver Valerius, Bastian Johnk, and Gerhard H. Braus 15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra Ry R. Forseth and Frank C. Schroeder 16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strau


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