MIDTAL (Microarrays for the Detection of Toxic Algae) presents protocols for microarray hybridization of toxic algae.
- Introduction
- Probe Design
- Field Sampling for Microarray Chip
- Preparation of Cultures for Analysis on the Chip
- Preparation of Controls
- RNA Extraction
- RNA Quantification Using the NanoDrop and Bioanalyzer
- Labeling of RNA using PlatinumBright 647 Infrared (KREAtech)
- Mircoarrays
- Scanning and Analysis of Microarray Slide
- Analysis of GPR Files
- The MIDTAL Database
- Toxin Microarray
- Probe List.- A collection of methods used in the detection of toxic algae