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Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins: Methods and Protocols. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins Karine Rousseau and Dallas M. Swallow
2. Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies Julia R. Davies, Claes Wickstrom and David J. Thornton
3. Detecting, Visualising and Quantifying Mucins Ceri A. Harrop, David J. Thornton and Michael A. McGuckin
4. Mass Spectrometric Analysis of Mucin Core Proteins Mehmet Kesimer and John K. Sheehan
5. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates Thomas A. Gerken
6. Analysis of Assembly of Secreted Mucins Malin E.V. Johansson and Gunnar C. Hansson
7. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey
8. Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunseng Jin, and Niclas G. Karlsson
9. O-Glycomics: Profoliling and Structural Analysis of Mucin-type O-linked Glycans Isabelle Breloy
10. O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry Franz-Georg Hanisch
11. Analysing Mucin Degradation Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
12. Assessment of Mucus Thickness and Production In Situ Lena Holm and Mia Phillipson
13. Preservation of Mucus in Histrological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH Malin E.V. Johansson and Gunnar C. Hansson
14. In vitro Measurements of Mucus Secretion by Colon Explants Jenny K. Gustafsson, Henrik Sjovall and Gunnar C. Hansson
15. Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and FunctionDavid R. Hill and Brian Button David R. Hill and Brian Button David R. Hill and Brian Button David R. Hill and Brian Button
16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis
17. Assessment of Intracellular Mucin Content In Vivo Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
18. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo Yong H. Sheng, Sumaira Z. Hasain, Chin Wen Png, Michael A. McGuckin and Sara K. Linden
19. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro Pablo Augueso and Ilene K. Gipson
