United States
£ GBP
In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.
Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, Transgenic Plants: Methods and Protocols, Second Edition encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.
Contents
Preface
Contributors
Part I. Selection AND DETECTION methods
1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation Craig M. Richael and Caius M. Rommens
2. Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed T. Scott Pinkerton, James R. Wild, and John A. Howard
3. Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants Basel Khraiwesh
Part II. Transformation Technology - Lower Plants
4. Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock
Part III. Transformation Technology - Rice
5. A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.) Kenjirou Ozawa
6. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene Masaki Endo, Tsutomu Shimizu and Seiichi Toki
7. Visual Selection in Rice-a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products Hiroaki Saika, Haruko Onodera and Seiichi Toki
8. Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui
9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui
Part IV. Transformation Technology - Cereals and Other Monocots
10. Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux
11. Split-transgene Expression in Wheat Mario Gils, Myroslava Rubtsova, and Katja Kempe
12. Agrobacterium-mediated Transformation of Brachypodium distachyon Vera Thole and Philippe Vain
13. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules Inger Baksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm
14. Biolistic Mediated Production of Transgenic Oil Palm Ghulam Kadir Ahmad Parveez and Bohari Bahariah
15. Transformation of Oil Palm using Agrobacterium tumefaciens Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani
Part V. Transformation Technology - Dicots
16. Highly Efficient Transformation Protocol for Plum (Prunus domestica L.) Cesar Petri, Ralph Scorza, and Chinnathambi Srinivasan
17. Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray
18. Initiation and Transformation of Grapevine Embryogenic Cultures Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray
19. Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless Mercedes Dabauza and Leonardo Velasco
20. Cotton Pistil Drip Transformation Method Tianzhen Zhang and Tianzhi Chen
21. Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton Tian-zhen Zhang and Shen-jie Wu
22. Targeted Biolistics for Improved Transformation of Impatiens balsamina Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi
23. A Protocol for Transformation of Torenia Ryutaro Aida
24. Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe
Part VI. Gene Targeting, Silencing and Directed Mutation
25. Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank
26. High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker
27. A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants Lilya Kopertekh, Inge Broer, and Joachim Schiemann
28. Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic Engineering Leandro H. Estornell, Antonio Granell, and Diego Orzaez
29. A One-time Inducible Transposon to Create Knockout Mutants in Rice Yuh-Chyang Charng
30. Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE) Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine
31. Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases Tonya L. Strange Moynahan and Joseph F. Petolino
32. Double-strand Break-induced Targeted Mutagenesis in Plants L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones
Part VII. Metabolic Engineering and Pharming
33. Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-Lopez, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou
34. Production of a His-Tagged Canecystatin in Transgenic Sugarcane Flavio Henrique-Silva and Andrea Soares-Costa
35. Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals Nunzia Scotti and Teodoro Cardi
36. Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein Yuhya Wakasa and Fumio Takaiwa
37. How to Grow Transgenic Arabidopsis in the Field Hanna Johansson Jankanpaa and Stefan Jansson
