Books  Animal & General Biology  Animals: General 

Key Experiments in Practical Developmental Biology

Handbook / Manual
Classic experiments in developmental biology described by the leaders in the field who originally carried out the research
Includes helpful information for course instructors such as potential sources of failure, key teaching concepts, sources of materials
Provides a bridge between research and what is learnt by advanced students in the field of developmental biology

By: Manuel Marí-Beffa (Editor), Jennifer Knight (Editor)

398 pages, 7 colour & 98 b/w illustrations, 12 tables

Cambridge University Press

Hardback | Mar 2005 | #210116 | ISBN: 0521833159
Temporarily out of stock: order now to get this when available Details
NHBS Price: £104.99 $132/€125 approx

About this book

Originally published in 2005, this unique resource Key Experiments in Practical Developmental Biology presents 27 easy-to-follow laboratory exercises for use in student practical classes in developmental biology. These experiments provide key insights into developmental questions, and many of them are described by the leaders in the field who carried out the original research. Key Experiments in Practical Developmental Biology intends to bridge the gap between experimental work and the laboratory classes taken at the undergraduate and post-graduate levels.

All chapters follow the same format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale and analysis employed in the research. Key Experiments in Practical Developmental Biology will be an invaluable resource for graduate students and instructors teaching practical developmental biology courses. Chapters include teaching concepts, discussion of the degree of difficulty of each experiment, potential sources of failure, as well as the time required for each experiment to be carried out in a class with students.


Contents

List of contributors
Preface Manuel Marí
Introduction Jennifer Knight

Part I. Graftings:
1. Two developmental gradients control head formation in hydra H. R. Bode
2. Embryonic regulation and induction in sea urchin development C. A. Ettensohn
3. The isthmic organizer and brain regionalization in chick embryos D. Echevaarría and S. Martínez

Part II. Specific Chemical Reagents:
4. Chemotaxis of aggregating Dictyostelium cells G. Gerisch and M. Ecke
5. Inhibition of signal transduction pathways prevents head regeneration in hydra L. M. Salgado
6. Retinoic acid during limb regeneration M. Maden

Part III. Beads Implantation:
7. Experimental techniques in avian embryos Y. Gañán, J. Rodríguez-León and D. Macías
8. Induction of ectopic limb outgrowth in chick with FGF-8 A. Raya, C. Rodríguez Esteban and J. C. Izpisúa-Belmonte

Part IV. Nucleic Acid Injections:
9. RNAi techniques applied to freshwater planarians (Platyhelminthes) during regeneration D. Bueno, R. Romero and E. Saló
10. Microinjection of Xenopus embryos R. J. Garriock and P. A. Krieg

Part V. Genetic Analysis:
11. Segmental specification in Drosophila melanogaster L. De Navas, M. Suzanne, D. Foronda and E. Sánchez-Herrero
12. Genetic analysis of flower development in Arabidopsis thaliana: the ABC model of floral organ identity determination J. L. Riechmann
13. Genetic analysis of vulva development in C. elegans S. Canevascini

Part VI. Clonal Analysis:
14. The role of the gene apterous in the development of the Drosophila wing F. J. Díaz-Benjumea
15. Extramacrochaetae, an example of a gene required for control of limb size and cell differentiation during wing morphogenesis in Drosophila A. Baonza
16. Hedgehog transduction pathway is involved in pattern formation in Drosophila melanogaster tergites M. Marí-Beffa

Part VII. In situ Hybridization:
17. Retinoic acid signalling controls anteroposterior patterning of the zebrafish hindbrain G. Begemann
18. Left-right asymmetry in the mouse M. Blum, A. Schweickert and C. Karcher

Part VIII. Transgenic Organisms:
19. Bicoid and Dorsal: two transcriptions factor gradients which specify cell fates in the early Drosophila embryo S. Roth
20. Significance of the temporal modulation of Hox gene expression on segment morphology J. Castelli-Gair Hombría
21. The UAS/GAL4 system for tissue-specific analysis of EGFR gene function in Drosophila melanogaster J. B. Duffy and N. Perrimon
22. Neurogenesis in Drosophila: a genetic approach C. Klämbt and H. Vaessin
23. Role of the achaete-scute complex genes in the development of the adult peripheral nervous system of Drosophila melanogaster S. Sotillos and S. Campuzano

Part IX. Vertebrate Cloning:
24. The conservation of the genome and nuclear reprogramming in Xenopus J. B. Gurdon

Part X. Cell Culture:
25. In vitro culture and differentiation of mouse embryonic stem cells A. Rolletschek, C. Wiese and A. M. Wobus

Part XI. Evo-Devo Studies:
26. Microevolution between Drosophila species N. Skaer and P. Simpson

Part XII. Computational Modelling:
27. Theories as a tool to understand the complex network of molecular interactions M. Meinhardt

Appendices
Index


Write a review

There are currently no reviews for this product. Be the first to review this product!


Biography

Manuel Mari-Beffa is a Lecturer in Developmental Biology at the University of Malaga. Jennifer Knight is an Instructor in the Department of Molecular, Cellular and Developmental Biology at the University of Colorado, Boulder.


Contributors:
- M. Marí-Beffa
- G. Begemann
- M. Blum
- A. Schweickert
- C. Karcher
- S. Roth
- J. Castelli-Gair Hombría
- J. B. Duffy
- N. Perrimon
- C. Klämbt
- H. Vaessin
- S. Sotillos
- S. Campuzano
- J. B. Gurdon
- A. Rolletschek
- C. Wiese
- A. M. Wobus
- N. Skaer
- P. Simpson
- M. Meinhardt- M. Marí-Beffa
- G. Begemann
- M. Blum
- A. Schweickert
- C. Karcher
- S. Roth
- J. Castelli-Gair Hombría
- J. B. Duffy
- N. Perrimon
- C. Klämbt
- H. Vaessin
- S. Sotillos
- S. Campuzano
- J. B. Gurdon
- A. Rolletschek
- C. Wiese
- A. M. Wobus
- N. Skaer
- P. Simpson
- M. Meinhardt
 

Bestsellers in this subject