Developmental Biology of the Sea Urchin and Other Marine Invertebrates Methods and Protocols

About this book

In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.

Contents

1. Culturing Larvae of Marine Invertebrates Richard R. Strathmann
2. Obtaining Gametes and Embryos of Ascidians Charles C. Lambert
3. Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis Anthony Pires
4. Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential Andreas Heyland, Roger Croll, Sophie Goodall, Jeff Kranyak, and Russell Wyeth
5. Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses Michael H. Temkin
6. An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images George von Dassow
7. TEM Analyses of Chaetognath Reproductive Organs George L. Shinn
8. SEM Analysis of Marine Invertebrate Gametes John Buckland-Nicks
9. Imaging Neural Development in Embryonic and Larval Sea Urchins Oliver Krupke, Shunsuke Yaguchi, Junko Yaguchi, and Robert D. Burke
10. Preparation and Use of Sea Urchin Egg Homogenates Anthony J. Morgan & Antony Galione
11. Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata Alex McDougall, Karen Wing-man Lee and Remi Dumollard
12. Isolating Specific Embryonic Cells of the Sea Urchin by FACS Celina Juliano, S. Zachary Swartz, and Gary Wessel
13. Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata Samantha Cihal and David Carroll
14. Perturbations to the Hedgehog Pathway in Sea Urchin Embryos Jacob F. Warner and David R. McClay
15. Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo Jolanta Kisielewska and Michael Whitaker
16. Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nemertean Worms Jose R. Escalona and Stephen A. Stricker
17. Multi-color Labeling in Developmental Gene Regulatory Network Analysis Aditya J. Sethi, Robert C. Angerer and Lynne M. Angerer
18. A 96-well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay Julio B. J. Harvey
19. Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines Michelle M. Roux and Kathy R. Foltz
20. Tools for Sea Urchin Genomic Analysis R. Andrew Cameron
21. Antibody Inhibition of Protein Activity in Starfish Oocytes Eiichi Okumura, Masatoshi Hara and Takeo Kishimoto
22. Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, and Tetsuo Toraya

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