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About this book
A clearly written, well-documented text which explains the requirements for particular applications and enables students to undertake experiments with a minimum of additional guidance.
Contents
Introduction and techniques: introductory history; laboratory organization; media; aseptic manipulation. Basic aspects: cell culture; cellular totipotency; somatic embryogenesis. Applications to plant breeding: haploid production; triploid production; in vitro pollination and fertilization; zygotic embryo culture; somatic hybridisation and cybridisation; genetic transformation; somaclonal and gametoclonal variant selection. Application to horticulture and forestry: production of disease-free plants; clonal propagation. General applications: industrial applications - secondary metabolite production; germplasm conservation.
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