Epidermal Cells Methods and Protocols

About this book

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Contents

1. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage Igor Kogut, Dennis R. Roop, and Ganna Bilousova
2. Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells Fahad K. Kidwai, Tong Cao, and Kai Lu
3. Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds Dongxia Ye and Antonio Peramo
4. Growth and Differentiation of HaCaT Keratinocytes Van G. Wilson
5. Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors Vincent P. Ramirez and Brian J. Aneskievich
6. Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq Jason M. Rizzo and Satrajit Sinha
7. Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg
8. Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline Raphaela Rid, Harald Hundsberger, and Kamil Onder
9. Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, and Wendy B. Bollag
10. Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes Kathleen P. McGuinn and My G. Mahoney
11. MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures Takashi Kobayashi
12. Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope Wilbert P. Vermeij and Claude Backendorf
13. Modified Methods for Growing 3-D Skin Equivalents: An Update Rebecca Lamb and Carrie A. Ambler
14. A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro Yoji Okugawa and Yohei Hirai
15. Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter Evelyne De Vuyst, Celine Charlier, Severine Giltaire, Valerie De Glas, Catherine Lambert de Rouvroit, and Yves Poumay
16. Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications John J. Wille, Jeremy J. Burdge, and Jong Y. Park
17. Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection Laura M. Griffin, Louis Cicchini, Tao Xu, and Dohun Pyeon
18. Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle Christine Chevalier, Jean-Francois Nicolas, and Anne-Cecile Petit
19. Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies Ines Sequeira, Emilie Legue, Suzanne Capgras, and Jean-Francois Nicolas
20. Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles Paula L. Miliani de Marval, Sun Hye Kim, and Marcelo L. Rodriguez Puebla
21. Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process Shinji Nakaoka

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