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Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes.
In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.
1. Reconstructing Integrin Activation in vitro Alexandre R. Gingras, Feng Ye, and Mark H. Ginsberg
2. Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy Jens Friedrichs, Carsten Werner, and Daniel J. Muller
3. A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics Warwick S. Nesbitt, Francisco J. Tovar-Lopez, Erik Westein, Ian S. Harper, and Shaun P. Jackson
4. Localization-Based Super-Resolution Imaging of Cellular Structures Pakorn Kanachanwong and Clare M. Waterman
5. Use of Microarray Analysis to Investigate EMT Gene Signatures Andy H. Sims, A.A. Larionov, D.J. Harrison, and Elad Katz
6. Podosome Reformation in Macrophages: Assays and Analysis Pasquale Cervero, Linda Panzer, and Stefan Linder
7. Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells Mirjam Andreasson-Ochsner and Erik Reimhult
8. Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay Marie Schoumacher, Alexandros Glentis, Vasily V. Gurchenkov, and Danijela M. Vignjevic
9. Cytoplasmic Actin: Purification and Single Molecule Assembly Assays Scott D. Hansen, J. Bradley Zuchero, and R. Dyche Mullins
10. Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity Aron Parekh,Jonathan M. Page, Scott A. Guelcher, and Alissa M. Weaver
11. Determining Rho GTPase Activity by an Affinity-Precipitation Assay Narendra Suryavanshi and Anne J. Ridley
12. Proteomic and Biochemical Methods to Study the Cytoskeletome Richard L. Klemke, Xinning Jiang, Sunkyu Choi, and Jonathan A. Kelber
13. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins Molly Lowndes and W. James Nelson
14. Purification of Native Arp2/3 Complex from Bovine Thymus Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
15. Purification of Arp2/3 Complex from Saccharomyces Cerevisiae Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
16. Measurement and Analysis of in vitro Actin Polymerization Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
17. Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions Simon Scrace, Eric O'Neill, Ester M. Hammond and Isabel M. Pires
18. Measuring Chemotaxis Using Direct Visualisation Microscope Chambers Andrew J Muinonen-Martin, David A. Knecht, Douwe M. Veltman, Peter A. Thomason, Gabriela Kalna, and Robert H. Insall
19. In Vitro Microtubule Severing Assays Natasza E. Ziolkowska and Antonina Roll-Mecak
20. VE-Cadherin Status as an Indicator of Microvascular Permeability Elizabeth Monaghan-Benson and Keith Burridge
21. High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis Ved P. Sharma, David Entenberg, and John Condeelis
22. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, and Louis Hodgson
23. Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion Joshua S. McLane, Nicholas J. Schaub, Ryan J. Gilbert, and Lee A. Ligon
24. Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap
