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Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.
Part I: Sampling, Chromatographic Purification, and Chemical Analysis of Pheromone Molecules
1. Female Moth Pheromones Tetsu Ando
2. Pheromones in the Fruit Fly Jacqueline Chin and Joanne Y. Yew
3. Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry Milos V. Novotny and Helena A. Soini
4. Murine Non-Volatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1 Hiroko Kimoto and Kazushige Touhara
5. Chemical Analysis of Aquatic Pheromones in Fish Michael Stewart, Cindy F. Baker, and Peter W. Sorensen
6. Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy Xinxing Zhang, Jaime H. Noguez, Yue Zhou, and Rebecca A. Butcher
Part II: Approaches to Characterize Gene and Function of Pheromone Receptors
7. Identification of Chemosensory Receptor Genes from Vertebrate Genomes Yoshihito Niimura
8. Functional Assays for Insect Olfactory Receptors in Xenopus Oocytes Tatsuro Nakagawa and Kazushige Touhara
9. A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami
10. Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes Tomohiro Ishii
Part III: Methods in Measuring Neural Responses to Pheromones
11. Electro-Antennogram and Single Sensillum Recording in Insect Antennae Shannon B. Olsson and Bill S. Hansson
12. Calcium Imaging of Pheromone Responses in the Insect Antennal Lobe Susy M. Kim and Jing W. Wang
13. Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons Angeldeep Kaur, Sandeepa Dey, and Lisa Stowers
14. Whole Mount Imaging of Responses in Mouse Vomeronasal Neurons Pei Sabrina Xu and Timothy E. Holy
15. Calcium Imaging of Vomeronasal Organ Response Using Slice Preparations from Transgenic Mice Expressing G-CaMP2 C. Ron Yu
16. The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ Trese Leinders-Zufall and Frank Zufall
17. Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations Julian P. Meeks and Timothy E. Holy
18. Pheromone-Induced Expression of Immediate Early Genes in the Mouse Vomeronasal Sensory System Sachiko Haga-Yamanaka and Kazushige Touhara
Part IV: Protocols in Analyzing Behavioral and Endocrine Changes Elicited by Pheromones
19. Insect Pheromone Behavior: Fruit Fly Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa
20. Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans Scott J. Neal, Kyuhyung Kim, and Piali Sengupta
21. Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion) Heeun Jang and Cornelia I. Bargmann
22. Behavioral Analysis of Pheromones in Fish Peter W. Sorensen
23. Analysis of Male Aggressive and Sexual Behavior in Mice Takefumi Kikusui
24. Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice Olivier Brock, Julie Bakker, and Michael J. Baum
25. Assessing Post-partum Maternal Care, Alloparental Behavior, and Infanticide in Mice: With Notes on Chemosensory Influences Kumi O. Kuroda and Yousuke Tsuneoka
26. Testing Smell When It is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse Benoist Schaal, Syrina Al Ain, and Bruno Patris
27. An Assay for Human Chemosignals Idan Frumin and Noam Sobel
