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Circadian Rhythms and Biological Clocks, Part A

Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers research methods in biomineralization science
Keeping with the interdisciplinary nature of the circadian rhythm field, the volume includes diverse approaches towards the study of rhythms, from assays of biochemical reactions in unicellular organisms to monitoring of behavior in humans
Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology
This volume also delves into the impact of circadian disruption on human health

Series: Methods in Enzymology Volume: 551

By: Amita Sehgal (Editor)

488 pages, illustrations

Academic Press

Hardback | Jan 2015 | #223476 | ISBN-13: 9780128012185
Availability: Usually dispatched within 1 week Details
NHBS Price: £121.00 $154/€143 approx

About this book

Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks, Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, Circadian Rhythms and Biological Clocks provides a wide range of assays for different biological systems.


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Circadian Rhythms and Biological Clocks, Part B

Two new volumes of Methods in Enzymology continue the legacy of this...

NHBS Price: £121.00 $154/€143 approx
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Contents

1. Studying Circadian Rhythm and Sleep Using Genetic Screens in Drosophila
Sofia Axelrod, Lino Saez and Michael W. Young
2. Dissecting the Mechanisms of the Clock in Neurospora
Jennifer Hurley, Jennifer J. Loros and Jay C. Dunlap
3. High Throughput and Quantitative Approaches for Measuring Circadian Rhythms in Cyanobacteria Using Bioluminescence
Ryan K. Shultzaberger, Mark L. Paddock, Takeo Katsuki, Ralph J. Greenspan and Susan S. Golden
4. Using Circadian Entrainment to Find Cryptic Clocks
Zheng Eelderink-Chen, Maria Olmedo, Jasper Bosman and Martha Merrow
5. Wavelet-based Analysis of Circ adian Behavioral Rhythms
Tanya L. Leise
6. Genetic Analysis of Drosophila Circadian Behavior in Semi-Natural Conditions
Edward W Green, Emma K O’Callaghan, Mirko Pegoraro, J Douglas Armstrong, Rodolfo Costa and Charalambos P Kyriacou
7. Methods to Study Molecular Mechanisms of the Neurospora Circadian Clock
Joonseok Cha, Mian Zhou, and Yi Liu
8. Detecting KaiC Phosphorylation Rhythms of the Cyanobacterial Circadian Oscillator in Vitro and in Vivo
Yong-Ick Kim, Joseph S. Boyd, Javier Espinosa, and Susan S. Golden
9. The Role of Casein Kinase I in the Drosophila Circadian Clock
Jeffrey L. Price, Jin-Yuan Fan, Andrew Keightley and John C. Means
10. Purification and Analysis of PERIOD Protein Complexes of the Mammalian Circadian Clock
Jin Young Kim, Pieter Bas Kwak, Michael Gebert, Hao A. Duong and Charles J. Weitz
11. Best Practices for Fluorescence Microscopy of the Cyanobacterial Circadian Clock
Susan E. Cohen, Marcella L. Erb, Joe Pogliano and Susan S. Golden
12. Structural and Biophysical Methods to Analyze Clock Function and Mechanism
Martin Egli
13. Identification of Small Molecule Modulators of the Circadian Clock
Tsuyoshi Hirota and Steve A. Kay
14. ChIP-Seq and RNA-Seq Methods to Study Circadian Control of Transcription in Mammals
Joseph S. Takahashi, Vivek Kumar , Prachi Nakashe, Nobuya Koike, Hung-Chung Huang, Carla B. Green, and Tae-Kyung Kim
15. Chipping away at the Drosophila Clock
Jian Zhou, Wangjie Yu and Paul E. Hardin
16. Considerations for RNA-seq Analysis of Circadian Rhythms
Jiajia Li, Gregory R Grant, John B Hogenesch and Michael E Hughes
17. RNA-Seq Profiling of Small Numbers of Drosophila Neurons
Katharine Abruzzi, Xiao Chen, Emi Nagoshi, Abby Zadina, and Michael Rosbash
18. Analysis of Circadian Regulation of Poly(A) Tail Length
Shihoko Kojima and Carla B. Green
19. Sample Preparation for Phosphoproteomic Analysis of Circadian Time Series in Arabidopsis thaliana
Johanna Krahmer, Matthew M Hindle, Sarah F Martin, Thierry Le Bihan and Andrew J Millar


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Biography

Dr. Sehgal is the John Herr Musser Professor of Neuroscience and Director of a Program in Chronobiology at the Perelman School of Medicine, University of Pennsylvania. She has also been an Investigator of the Howard Hughes Medical Institute since 1997. Dr. Sehgal received her Ph.D. from the Graduate School of Medical Sciences, Cornell University, working with Dr. Moses Chao, and conducted her postdoctoral work with Dr. Michael Young at Rockefeller University. A major focus in her laboratory is the cellular and molecular basis of circadian rhythms, using the fruit fly, Drosophila melanogaster as a model system. Her laboratory also developed a Drosophila model for the study of sleep, which has been adopted by laboratories worldwide, and is rapidly provding insight into the regulation and function of sleep. In 2009, Dr. Sehgal was elected to the Institute of Medicine and in 2011 to the American Academy of Arts and Sciences.

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