The rapid development of technologies targeting at the analysis of genomic resources (next-generation sequencing) has revolutionized biological sciences. For biological systematics and biodiversity research, the application of molecular markers has opened new avenues for understanding phylogeny, evolution and functions, for discovering and classifying biodiversity. Nowadays, NGS technologies offer novel tools for increasing both quantity and quality of molecular data. The applicability to non-model organisms provides links to molecular biology and plant breeding. Next-Generation Sequencing in Plant Systematics reviews current issues in the application of NGS technologies in phylogenetic, evolutionary, biodiversity and population genetic studies. The focus is on plants, but most chapters also address animals and fungi. After a brief introduction, the topics contributed by international experts include organellar genomics, transcriptome sequencing for phylogenetic studies, polyploid genomes, asexual genome evolution, repetitive DNA, restriction site associated DNA (RADseq), microsattelite discovery, NGS on plant herbarium tissues. The focus on methodologies is of interest for advanced students, researchers and scientific lecturers in all fields of biology. Next-Generation Sequencing in Plant Systematics will be of special interest for reserachers in plant systematics, plant evolutionary biology, population generics, biodiversity research, conservation genetics, and plant breeding. The focus of the contributions is on case studies on non-model organisms, for which completely sequenced reference genomes are usually not yet available.
- Elvira Hörandl & Marc Appelhans: Introduction to chapters and methodological overview
- Susann Wicke & Gerald M. Schneeweiss: Next-generation organellar genomics: Potentials and pitfalls of high-throughput technologies for molecular evolutionary studies and plant systematics
- Jun Wen, Ashley N. Egan, Rebecca B. Diekow & Elizabeth A. Zimmer: Utility of transcriptome sequencing for phylogenetic inference of character evolution
- Armel Salmon & Malika Ainouche: Next-generation sequencing and the challenge of deciphering evolution of recent and highly polyploid genomes
- Diego Hojsgaard, M. Pellino, T. Sharbel & E. Hörandl: Resolving genome evolution patterns in asexual plants
- Hanna Weiss-Schneeweiss, Andrew R. Leitch, Jamie McCann, Tae-Soo Jang & Jiri Macas: Employing next generation sequencing to explore the repeat landscape of the plant genome
- Richard H. Ree and Andrew L. Hipp: Inferring phylogenetic history from restriction site associated DNA (RADseq)
- Kurt Weising, Tina Wöhrmann & Bruno Huettel: The use of high-throughput DNA sequencing for microsattelite discovery in plants
- Fred T. Bakker: DNA sequences from plant herbarium tissue
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