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PCR Protocols: A Guide to Methods and Applications

Handbook / ManualOut of Print

Edited By: MA Innis

482 pages, B/w photos, figs

Academic Press

Spiralbound | Dec 1990 | #25397 | ISBN: 0123721814
Out of Print Details

About this book

The polymer chain reaction (PCR) is a powerful new molecular biological method with widespread applications in genetics. This volume addresses the complete range of PCR methods and research applications.

For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols, A Guide to Methods and Applications is an invaluable reference. This work must, by its nature, be definitive; the first three editors are from Cetus, the corporation... The editors have done well to give this work a surprisingly cohesive feel... This manual is an excellent summary of PCR to date. The index is good and the reference citations alone would save an investigator new to the field countless hours in the library. The prose and 'cookbook' presentation of the procedures are clear at an appropriate level for anyone with a background in more conventional molecular techniques. While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete. --APPLIED CYTOGENETICS "PCR Protocols is an excellent lab manual for making PCR work... Most of the articles are written with numbered, step-by-step procedures and have reagent information, down to the sequences of the oligonucleotides used. Even the comb binding of the book makes it suitable for bench-top use... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... The 30 or so applications articles give the experienced reader a good overview of the power and utility of PCR. In summary, if you have questions about PCR, PCR Protocols probably has the answers." --TIG "This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR...This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR...No user of the PCR technique could wish to miss it." --TIBTECH "The fifty-five chapters presented in this guide to the methods and applications of PCR provide detailed experimental protocols. They are written by researchers who use PCR successfully in their laboratory and who do not shy away from pointing out the potential pitfalls. This extremely useful publication of PCR protocols goes far beyond the simple amplification of nucleic acids... A well-organized index is provided. This book will make it more difficult to find an excuse for not using the PCR technique." --AMERICAN JOURNAL OF PHYSIOLOGY


Contents

Basic Methodology: M.A. Innis and D.H. Gelfand, Optimization of PCRs. R.K. Saiki, Amplification of Genomic DNA. E.S. Kawasaki, Amplification of RNA. M.A. Frohman, RACE: Rapid Amplification of cDNA Ends. T. Compton, Degenerate Primers for DNA Amplification. C.C. Lee and C.T. Caskey, cDNA Cloning Using Degenerate Primers. M.A. Innis, PCR with 7-Deaza-2#b7-Deoxyguanosine Triphosphate. G. Gilliland, S. Perrin, and H.F. Bunn, Competitive PCR for Quantitation of mRNA. A.M. Wang and D.F. Mark, Quantitative PCR. P.C. McCabe, Production of Single-Stranded DNA by Asymmetric PCR. S.J. Scharf, Cloning with PCR. U. Landegren, R. Kaiser, and L. Hood, Oligonucleotide Ligation Assay. C. Levenson and C.-A. Chang, Nonisotopically Labeled Probes and Primers. Y-M.D. Lo, W.Z. Mehal, and K.A. Fleming, Incorporation of Biotinylated dUTP. R. Helmuth, Nonisotopic Detection of PCR Products. D.H. Gelfand and T.J. White, Thermostable DNA Polymerases. S. Kwok, Procedure to Minimuze PCR-Product Carry-Over. E.S. Kawasaki, Sample Preparation from Blood, Cells, and Other Fluids. D.K. Wright and M.M. Manos, Sample Preparation from Paraffin-Embedded Tissues. S. P#ada#adabo, Amplifying Ancient DNA. Research Applications. M.J. Holland and M.A. Innis, In Vitro Transcription of PCR Templates. R. Higuchi, Recombinant PCR. B. Krummel, DNase I Footprinting. M.A.D. Brow, Sequencing with Taq DNA Polymerase. S.S. Sommer, G. Sarkar, D.D. Koeberl, C.D.K. Bottema, J.-M. Buerstedde, D.B. Schowalter, and J.D. Cassady, Direct Sequencing with the Aid of Phage Promoters. V.C. Sheffield, D.R. Cox, and R.M. Myers, Identifying DNA Polymorphisms by Denaturing Gradient Gel Electrophoresis. H. Ochman, M.M. Medhora, D. Garza, and D.L. Hartl, Amplification of Flanking Sequences by Inverse PCR. M.A. Frohman and G.R. Martin, Detection of Homologous Recombinants. L.M. Powell, RNA Processing: Apo-B. T.R. Gingeras, G.R. Davis, K.M. Whitfield, H.L. Chappelle, L.J. DiMichele, and D.Y. Kwoh, A Transcription-Based Amplification System. K.D. Friedman, N.L. Rosen, P.J. Newman, and R.R. Montgomery, Screening of #glgt11 Libraries. Genetics and Evolution. H.A. Erlich and T.L. Bugawan, HLA DNA Typing. J.S. Chamberlain, R.A. Gibbs, J.E. Ranier, and C.T. Caskey, Multiplex PCR for the Diagnosis of Duchenne Muscular Dystrophy. S.B. Lee and J.W. Taylor, Isolation of DNA from Fungal Mtcelia and Single Spores. S.C. Kogan and J. Gitschier, Genetic Prediction of Hemophilia A. U. Gyllensten, Haplotype Analysis from Single Sperm or Diploid Cells. M.L. Sogin, Amplification of Ribosomal RNA Genes for Molecular Evolution Studies. T.J. White, T. Bruns, S. Lee, and J. Taylor, Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. Diagnostics and Forensics. G.D. Ehrlich, S. Greenberg, and M.A. Abbott, Detection of Human T-Cell Lymphoma/Leukemia Viruses. D.E. Kellogg and S. Kwok, Detection of Human Immunodeficiency Virus. I. Baginski, A. Ferrie, R. Watson, and D. Mack, Detection of Hepatitis B Virus. Y. Ting and M.M. Manos, Detection and Typing of Genital Human Papillomaviruses. D. Shibata, Detection of Human Cytomegalovirus. H.A. Rotbart, PCR Amplification of Enteroviruses. D. Mack, O.-S. Kwon, and F. Faloona, Novel Viruses. J. Lyons, Analysis of ras Gene Point Mutations by PCR and Olgonucleotide Hybridization. M. Crescenzi, B-Cell Lymphoma: t(14;18) Chromosome Rearrangement. R.M. Atlas and A.K. Bej, Detecting Bacterial Pathogens in Environmental Water Samples by Using PCR and Gene Probes. S.-H. Park, PCR in the Diagnosis of Retinoblastoma. C. Orrego and M.C. King, Determination of Familial Relationships. Instrumentation and Supplies: R. Watson, PCR in a Teacup A Simple and Inexpensive Method for Thermocycling PCRs. P. Denton and H. Reisner, A Low-Cost Air-Driven Cycling Oven. N.C.P. Cross, N.S. Foulkes, D. Chappel, J. McDonnell, and L. Luzzatto, Modification of a Histokinette for Use as an Automated PCR Machine. C. Orrego, Organizing a Laboratory for PCR Work. R. Madej and S. Scharf, Basic Equipment and Supplies. Index.

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