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PCR: A Practical Approach, Volume 1

Handbook / Manual

Series: PCR: A Practical Approach Volume: 1

Edited By: MJ McPherson, P Quirke and GR Taylor

IRL Press

Paperback | Sep 1991 | #25399 | ISBN: 0199631964
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £70.00 $89/€84 approx

About this book

The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls. This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.

This volume should be useful for those who have some experience in the PCR method and wish to extend the technology to more sophisticated and newer applications. --American Journal of Physiology
"The text and the procedures in these chapters and throughout the book are clearly written and often aided by the use of graphics and photographs of key elements. . . . an excellent presentation and organization of procedureal materials related to PCR." --Journal of the American Chemical Society


Contents

Graham R. Taylor: Polymerase chain reaction: basic principles and automation; Adrian Ivinson, & Graham R. Taylor: Polymerase chain reaction in genetic diagnosis; David P. Jackson, Julie D. Hayden, & Philip Quirke: Extraction of nucleic acid from fresh and archival material; Roland G. Roberts, A. Jane Montandon, Peter M. Green, & David R. Bentley: Analysis of genomic sequence variation using amplification and mismatch detection; Belinda J.F. Rossiter, Markus Grompe, & C. Thomas Caskey: Detection of deletions and point mutations; Michael Litt: PCR of TG microsatellites; Susan A. Ledbetter, & David L. Nelson: Genome amplification using primers directed to interspersed repetitive sequences (IRS-PRC); Daniel H. Johnson: PCR amplification of microdissected DNA; Jonathon Silver: Inverse polymerase chain reaction; Sarah Jane Gurr, & Michael J. McPherson: PCR-directed cDNA libraries; Michael J. McPherson, Kerrie M. Jones, & Sarah Jane Gurr: PCR with highly redundant primers; Tim Clackson, Detlef Gussow, & Peter T. Jones: General application of PCR to gene cloning and manipulation; Margaret J. Dallman, & Andrew C.G. Porter: Semi-quantitative PCR for the analysis of gene expression; Kirstin A. Eckert, & Thomas A. Kunkel: The fidelity of DNA polymerases used in the PCR; Appendix, Index

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