Books  Animal & General Biology  Genetics 

Techniques for the Analysis of Complex Genomes

Handbook / ManualOut of Print

Edited By: R Anand

240 pages, B/w photos, figs

Academic Press

Spiralbound | Dec 1992 | #26165 | ISBN: 0120576201
Out of Print Details

About this book

Describes genomic mapping by pulsed field gel electrophoresis, and provides a detailed account of the cloning of large fragments of DNA using yeast artificial chromosomes (YACs) and bacteriophage PI cloning systems.


YAC Library Construction and Vectors. Mapping Clones and Localization of Probes. YACs as Probes for cDNA Identification (Cloning of Islands). Homologous Recombination (Integration of Vectors, Neo Targetting). Building YACs Host Stains and Stability. Cloning Telomers. Olgio and Genome Mapping. P1 Cloning. Microdisection. Telomer Structure and Overview. Genome PFGE. SDS Approach to Olgio and Genome Mapping.

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