Books  Animal & General Biology  Biochemistry & Molecular Biology 

Basic Methods in Molecular Biology

TextbookHandbook / ManualOut of Print

By: L Davis, WM Kuehl and JF Battey

Prentice-Hall (Pearson Education)

Spiralbound | Dec 1994 | Edition: 2 | #40751 | ISBN: 0838506429
Out of Print Details

About this book

Revised edition of this standard manual.


General methods; bacterial strains and cloning vectors; enzymes that modify DNA and RNA; in vitro amplification of DNA using - the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction; DNA restriction fragment analysis and preparation, introduction; in vitro labeling of probes and filter hybridization; plasmid DNA preparation for E. Coli hosts; preparation of DNA from Lambda bacteriophage clones; subcloning fragments into plasmid vectors and plasmid construction; preparation of genomic DNA; preparation and analysis of RNA from eukaryotic cells - overview; geonomic cloning; generatino of cDNA libaries; preparation of subtractive cDNA, introduction; chain termination sequencing; transfection of mammalian cells, introduction; basic methods of protein analysis; in situ hybridization; transgenic mouse preparation; detection and in vitro generation of specific mutations in genes and cDNAs, introduction; appendices.

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