Recombinant DNA methods are powerful, revolutionary techniques for at least two reasons. First, they allow the isolation of single genes in large amounts from a pool of thousands or millions of genes. Second, the isolated genes or their regulatory regions can be modified at will and re-introduced into cells for expression at the RNA or protein levels. These attributes help to solve complex biological problems and to produce new and better products in the areas of health, agriculture and industry. Both volume 154 and 155 include the descriptions of several useful new restriction enzymes and rapid methods for DNA sequence analysis, along with a number of other useful methods.
Praise for the Volume "This reviewer finds Methods in Enzymology Volume 154, Recombinant DNA Part E to be excellent reference book for persons working in molecular biology." --ADOLESCENT AND PEDIATRIC GYNECOLOGY Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY
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